Antibody:	anti-Spag1 (98146)
Detection system:	Secondary antibody coupled to Cy3

Structure	Level	Pattern	Image	Note
TS28: oocyte of primordial follicle	Present	Regionally restricted	1A Pmf	Expression localized to cytoplasm of primary oocytes in primordial follicles.

Structure	Level	Pattern	Image	Note
TS28: oocyte of preantral follicle	Present	Regionally restricted	1A Pre-anf (left)	Expression localized in cytoplasm of oocytes in preantral follicles.

Structure	Level	Pattern	Image	Note
TS28: oocyte of preantral follicle	Present	Regionally restricted	1A Pre-anf (middle)	Authors report accumulation of expression in cortex.

Structure	Level	Pattern	Image	Note
TS28: oocyte of preantral follicle	Present	Regionally restricted	1A Pre-anf (right)	v

Structure	Level	Pattern	Image	Note
TS28: oocyte of antral follicle	Present	Diffuse	1A Anf	Expression diffused across cytoplasm of BV-stage oocytes in antral follicles.

Structure	Level	Pattern	Image	Note
TS28: primary oocyte	Present	Regionally restricted	1B GV	Expression accumulated as cytoplasmic dots around nuclei, with some foci attached to germinal vesicle.

Structure	Level	Pattern	Image	Note
TS28: secondary oocyte	Present		1B GVBD

Structure	Level	Pattern	Image	Note
TS28: premeiotic germ cell of ovary	Present	Regionally restricted	1B Pre-MI	After germinal vesicle breakdown, expression enriched in close proximity of chromosomes, tightly associated with mitotic spindles until metaphase II.

Structure	Level	Pattern	Image	Note
TS28: oocyte	Present	Regionally restricted	1B MI	After germinal vesicle breakdown, expression enriched in close proximity of chromosomes, tightly associated with mitotic spindles until metaphase II.

Structure	Level	Pattern	Image	Note
TS28: oocyte	Present	Regionally restricted	1B Ana/Tel I (upper)	After germinal vesicle breakdown, expression enriched in close proximity of chromosomes, tightly associated with mitotic spindles until metaphase II.

Structure	Level	Pattern	Image	Note
TS28: oocyte	Present	Regionally restricted	1B Ana/Tel I (lower)	After germinal vesicle breakdown, expression enriched in close proximity of chromosomes, tightly associated with mitotic spindles until metaphase II.

Structure	Level	Pattern	Image	Note
TS28: secondary oocyte	Present	Regionally restricted	1B MII	Expression maintained mincrotubule-associated localiztion during final steps of meiosis II.

Structure	Level	Pattern	Image	Note
TS28: secondary oocyte	Present		1B Ana/Tel II

Structure	Level	Pattern	Image	Note
TS1: 1-cell stage embryo	Present	Regionally restricted	1B PN	Expression evenly dispersed into cytoplasm of pronucleus-stage zygotes.

Structure	Level	Pattern	Image	Note
TS1: 1-cell stage embryo	Present	Regionally restricted	1B 1-cell (upper)	Expression reassembles to overlap with mitotic spindles in one-cell embryos

Structure	Level	Pattern	Image	Note
TS1: 1-cell stage embryo	Present	Regionally restricted	1B 1-cell (lower)	Expression reassembles to overlap with mitotic spindles in one-cell embryos

Structure	Level	Pattern	Image	Note
TS2: 2-cell stage embryo	Present	Regionally restricted	1B 2-cell	Expression predominantly diffused in cytoplasm in interphase cells and spindle-associated in dividing cells.

Structure	Level	Pattern	Image	Note
TS3: 4-cell stage embryo	Present	Regionally restricted	1B 4-cell	Expression predominantly diffused in cytoplasm in interphase cells and spindle-associated in dividing cells.

Structure	Level	Pattern	Image	Note
TS3: 8-cell stage embryo	Present	Regionally restricted	1B 8-cell	Expression predominantly diffused in cytoplasm in interphase cells and spindle-associated in dividing cells.

Structure	Level	Pattern	Image	Note
TS4: blastocyst	Present		1B BC