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Gene Expression Data
Assay Details
Assay
Reference: J:312371 Da Silva F, et al., Mitotic WNT signalling orchestrates neurogenesis in the developing neocortex. EMBO J. 2021 Oct 1;40(19):e108041
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7643180
Gene symbol: Eomes
Gene name: eomesodermin
Antibody: anti-Eomes (Dan11mag, 50-4875-82,14-4875-80)
Detection system: Secondary antibody
Results
Specimen 2G Co: embryonic day 13.5; Ccnytm1.1Smoc/Ccny+, Ccnyl1tm1a(EUCOMM)Wtsi/Ccnyl1+ (more )
Note: Triple labeled: blue - Eomes; green - IdU; red - BrdU.
Structure Cell Type Level Pattern Image Note
TS21: cerebral cortex subventricular zone neural progenitor cell Present 2G Co Expressed in basal progenitors.
Specimen 2G DKO: embryonic day 13.5; Ccnytm1.1Smoc/Ccnytm1.1Smoc, Ccnyl1tm1a(EUCOMM)Wtsi/Ccnyl1tm1a(EUCOMM)Wtsi (more )
Note: Triple labeled: blue - Eomes; green - IdU; red - BrdU.
Structure Cell Type Level Pattern Image Note
TS21: cerebral cortex subventricular zone neural progenitor cell Present 2G DKO Expressed in basal progenitors. A small increase in S-phase length of apical progenitors (Tbr2/Eomes-), and a 50% increase in S-phase length of positive basal progenitors compared with the control (quantified in Fig H, I).
Specimen 2K Co: embryonic day 13.5; Ccnytm1.1Smoc/Ccny+, Ccnyl1tm1a(EUCOMM)Wtsi/Ccnyl1+ (more )
Note: Triple labeled: blue - Eomes; green - Mki67 (assay MGI:7643223); red - pHH3.
Structure Cell Type Level Pattern Image Note
TS21: cerebral cortex subventricular zone neural progenitor cell Present 2K Co Expressed in basal progenitors.
Specimen 2K DKO: embryonic day 13.5; Ccnytm1.1Smoc/Ccnytm1.1Smoc, Ccnyl1tm1a(EUCOMM)Wtsi/Ccnyl1tm1a(EUCOMM)Wtsi (more )
Note: Triple labeled: blue - Eomes; green - Mki67 (assay MGI:7643223); red - pHH3.
Structure Cell Type Level Pattern Image Note
TS21: cerebral cortex subventricular zone neural progenitor cell Present 2K DKO Expressed in basal progenitors. No major differences in the percentage of cycling (Mki67+) apical progenitors (Tbr2-) and basal progenitors (Tbr2+) that were in mitosis (pHH3+).
Specimen Ev5 D: embryonic day 13.5 (more )
Note: Triple labeled: red - Eomes; green - Sox4 (assay MGI:7643183); magenta - pHH3.
Structure Cell Type Level Pattern Image Note
TS21: cerebral cortex subventricular zone neural progenitor cell Present EV5_D right , EV5_D middle Mainly expressed in basal progenitors.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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06/12/2024
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