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Gene Expression Data
Assay Details
Assay
Reference: J:195264 Vincentz JW, et al., Twist1 Controls a Cell-Specification Switch Governing Cell Fate Decisions within the Cardiac Neural Crest. PLoS Genet. 2013 Mar;9(3):e1003405
Assay type: RNA in situ
MGI Accession ID: MGI:7643418
Gene symbol: Phox2b
Gene name: paired-like homeobox 2b
Probe: Phox2b probe
Probe preparation: Antisense labelled with digoxigenin RNA
Results
Specimen 2E: embryonic day 11.5 (more )
Structure Cell Type Level Pattern Image Note
TS19: outflow tract endocardial cushion neural crest cell Absent 2E
Specimen 2F: embryonic day 11.5; H2az2Tg(Wnt1-cre)11Rth/0, Twist1tm1Bhr/Twist1tm2Bhr (more )
Note: Conditional mutant.
Structure Cell Type Level Pattern Image Note
TS19: outflow tract endocardial cushion neural crest cell Present Regionally restricted 2F Conditional ablation of Twist1 produces abnormal cellular aggregates. Expression of Phox2b was detected in the aggregates.
Specimen 6A: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: outflow tract endocardial cushion Absent 6A
Specimen 6B: embryonic day 11.5; Twist1Ska10/Twist1+ (more )
Structure Level Pattern Image Note
TS19: outflow tract Present Regionally restricted 6B Heterozygous outflow tracts contain dispersed or relatively small aggregates of Phox2b-positive cells.
TS19: outflow tract endocardial cushion Present Regionally restricted 6B Ectopic neurons were dispersed in the aorticopulmonary cushion ectomesenchyme.
Specimen 6C: embryonic day 11.5; Twist1Ska10/Twist1Ska10 (more )
Structure Level Pattern Image Note
TS19: outflow tract Strong Regionally restricted 6C Twist1<CC/CC> embryos contain robust ectopic Phox2b-positive ganglia.
TS19: outflow tract endocardial cushion Present Regionally restricted 6C Ectopic neurons were detected in the aorticopulmonary cushion.
Specimen 8J: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: thoracic ganglion Present 8J Expression was detected in the thoracic sympathetic chain ganglia.
Specimen S3A: embryonic day 10.5 (more )
Structure Level Pattern Image Note
TS17: aortico-pulmonary spiral septum Absent S3A
TS17: outflow tract endocardial cushion Absent S3A
Specimen S3B: embryonic day 10.5; H2az2Tg(Wnt1-cre)11Rth/0, Twist1tm1Bhr/Twist1tm2Bhr (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS17: aortico-pulmonary spiral septum Present Regionally restricted S3B Ectopic expression was observed in the conditional mutant at E10.5.
TS17: outflow tract endocardial cushion Present Regionally restricted S3B Ectopic expression was observed in the conditional mutant at E10.5.
Specimen S3E: embryonic day 16.5 (more )
Structure Level Pattern Image Note
TS24: cardiac muscle tissue Absent S3E
Specimen S3F: embryonic day 16.5 (more )
Structure Level Pattern Image Note
TS24: arch of aorta Absent S3F
Specimen S3G: embryonic day 16.5; H2az2Tg(Wnt1-cre)11Rth/0, Twist1tm1Bhr/Twist1tm2Bhr (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS24: cardiac muscle tissue Present Regionally restricted S3G Ectopic expression was detected in the myocardial cuff of the conditional mutant.
Specimen S3H: embryonic day 16.5; H2az2Tg(Wnt1-cre)11Rth/0, Twist1tm1Bhr/Twist1tm2Bhr (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS24: arch of aorta Present Regionally restricted S3H Ectopic expression was detected in the aortic arch of the conditional mutant.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Send questions and comments to User Support. 		last database update
10/09/2024
MGI 6.24 		The Jackson Laboratory