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Gene Expression Data
Assay Details
Assay
Reference: J:195264 Vincentz JW, et al., Twist1 Controls a Cell-Specification Switch Governing Cell Fate Decisions within the Cardiac Neural Crest. PLoS Genet. 2013 Mar;9(3):e1003405
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7643421
Gene symbol: Th
Gene name: tyrosine hydroxylase
Antibody: Anti-Th
Detection system: Secondary antibody coupled to DyLight
Results
Specimen 2K: embryonic day 11.5 (more )
Structure Cell Type Level Pattern Image Note
TS19: outflow tract endocardial cushion neural crest cell Absent 2K
Specimen 2L: embryonic day 11.5; H2az2Tg(Wnt1-cre)11Rth/0, Twist1tm1Bhr/Twist1tm2Bhr (more )
Note: Conditional mutant.
Structure Cell Type Level Pattern Image Note
TS19: outflow tract endocardial cushion neural crest cell Present Regionally restricted 2L Conditional ablation of Twist1 produces abnormal cellular aggregates. Expression of Th was detected in the aggregates.
Specimen 3A: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: outflow tract endocardial cushion Absent 3A
Specimen 3B: embryonic day 11.5; Twist1tm1Bhr/Twist1tm2Bhr, Hand1tm1.1(EGFP/cre)Abfi/Hand1+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS19: outflow tract endocardial cushion Present Regionally restricted 3B Conditional ablation of Twist1 produces abnormal cellular aggregates. Expression of Th was detected in the aggregates.
Specimen 6D: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: outflow tract endocardial cushion Absent 6D
Specimen 6E: embryonic day 11.5; Twist1Ska10/Twist1+ (more )
Structure Level Pattern Image Note
TS19: outflow tract endocardial cushion Present Single cells 6E Rare immunopositive cells were observed.
Specimen 6F: embryonic day 11.5; Twist1Ska10/Twist1Ska10 (more )
Structure Level Pattern Image Note
TS19: outflow tract endocardial cushion Present Regionally restricted 6F Twist1<CC/CC> embryos contain a greater number of immunopositive neurons.
Specimen 8A: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: thoracic ganglion Present 8A Expression was detected in the thoracic sympathetic chain ganglia.
Specimen S3M: embryonic day 16.5 (more )
Structure Level Pattern Image Note
TS24: cardiac muscle tissue Absent S3M
Specimen S3N: embryonic day 16.5 (more )
Structure Level Pattern Image Note
TS24: arch of aorta Absent S3N
Specimen S3O: embryonic day 16.5; H2az2Tg(Wnt1-cre)11Rth/0, Twist1tm1Bhr/Twist1tm2Bhr (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS24: cardiac muscle tissue Present Regionally restricted S3O Ectopic expression was detected in the myocardial cuff of the conditional mutant.
Specimen S3P: embryonic day 16.5; H2az2Tg(Wnt1-cre)11Rth/0, Twist1tm1Bhr/Twist1tm2Bhr (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS24: arch of aorta Present Regionally restricted S3P Ectopic expression was detected in the aortic arch of the conditional mutant.
Specimen not shown Control: embryonic day 11.5 (more )
Structure Cell Type Level Pattern Image Note
TS19: sympathetic ganglion sympathetic noradrenergic neuron Present
TS19: outflow tract endocardial cushion Absent
Specimen not shown Tw1fx/-;H2fx/+;Wnt1-Cre(+): embryonic day 11.5; Hand2tm1Cse/Hand2+, Twist1tm1Bhr/Twist1tm2Bhr, H2az2Tg(Wnt1-cre)11Rth/0 (more )
Note: Conditional mutant.
Structure Cell Type Level Pattern Image Note
TS19: sympathetic ganglion sympathetic noradrenergic neuron Present
TS19: outflow tract endocardial cushion Present Regionally restricted Expression was detected in ectopic sympathetic-like neurons.
Specimen not shown Tw1fx/+;H2fx/-;Wnt1-Cre(+): embryonic day 11.5; H2az2Tg(Wnt1-cre)11Rth/0, Hand2tm1Cse/Hand2tm1Dsr, Twist1tm2Bhr/Twist1+ (more )
Note: Conditional mutant.
Structure Cell Type Level Pattern Image Note
TS19: sympathetic ganglion sympathetic noradrenergic neuron Present Reduced expression was observed compared with control.
TS19: outflow tract endocardial cushion Absent
Specimen not shown Tw1fx/-;H2fx/-;Wnt1-Cre(+): embryonic day 11.5; Hand2tm1Cse/Hand2tm1Dsr, Twist1tm1Bhr/Twist1tm2Bhr, H2az2Tg(Wnt1-cre)11Rth/0 (more )
Note: Conditional mutant.
Structure Cell Type Level Pattern Image Note
TS19: sympathetic ganglion sympathetic noradrenergic neuron Absent
TS19: outflow tract endocardial cushion Present Regionally restricted Expression was detected in ectopic sympathetic-like neurons.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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10/09/2024
MGI 6.24 		The Jackson Laboratory