Antibody:	Anti-NR2F2 [EPR18443] (ab211777)
Detection system:	Secondary antibody coupled to Alexa Fluor 594

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present	Regionally restricted	3A WT	Expressed in cells localized at the upper layers, consisting with the distribution of glymphatic vessels.
TS28: glymphatic system	Present	Regionally restricted	3A WT	Expressed in cells localized at the upper layers, consisting with the distribution of glymphatic vessels.

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present		3A KO	A much deeper distribution and bigger number of positive cells were observed.
TS28: glymphatic system	Present		3A KO	A much deeper distribution and bigger number of positive cells were observed.

Structure	Level	Pattern	Image	Note
TS28: epidermis	Present		4A WT	Positive cells localized mainly in the epidermal layer.

Structure	Level	Pattern	Image	Note
TS28: skin	Present		4A KO	Positive cells expanded beyond epidermal layer and even into mesenchyme layers.
TS28: epidermis	Present		4A KO	More positive cells compared with the wild-type.