Gene Expression Data
Assay Details
Assay
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Reference:
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J:84852
Imanaka-Yoshida K, et al., The dynamic expression of tenascin-C and tenascin-X during early heart development in the mouse. Differentiation. 2003 Jun;71(4-5):291-8
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7645233
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Gene symbol:
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Tnxb
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Gene name:
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tenascin XB
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Antibody:
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anti-TNX
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Detection system:
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horseradish peroxidase
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Results
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Specimen
3B E11.0:
embryonic day 11.0
(more )
Specimen
3B E11.0:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 11.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Structure |
Level |
Pattern |
Image |
Note |
TS18: heart |
Present |
Scattered |
3B E11.0
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Scattered small spots of expression along interventricular groove and AV groove.
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TS18: heart atrium |
Present |
Regionally restricted |
3B E11.0
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Expression over surface of atrium.
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Specimen
3B E11.5:
embryonic day 11.5
(more )
Specimen
3B E11.5:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 11.5
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Sex:
|
Not Specified
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Type:
|
whole mount
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Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: heart |
Present |
Spotted |
3B E11.5
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Spots of expression increased and number and fuse with each other to form network.
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Specimen
3B E12.0:
embryonic day 12.0
(more )
Specimen
3B E12.0:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 12.0
|
Sex:
|
Not Specified
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Type:
|
whole mount
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Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: heart |
Present |
Regionally restricted |
3B E12.0
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Expression eventually covers entire surface of heart.
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Specimen
3C:
embryonic day 11.5
(more )
Specimen
3C:
(close )
Genetic Background:
|
Not Specified
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Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS19: visceral pericardium |
mesothelial cell of epicardium |
Present |
Regionally restricted |
3C
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Expression around epicardial cells forming vascular channels.
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Specimen
3D:
embryonic day 12.5
(more )
Specimen
3D:
(close )
Genetic Background:
|
Not Specified
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Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS20: ventricle cardiac muscle |
Present |
Regionally restricted |
3D
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Expression assocated with cells invading ventricular myocardium.
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Specimen
3H:
embryonic day 12.5
(more )
Specimen
3H:
(close )
Genetic Background:
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Not Specified
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Age:
|
embryonic day 12.5
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Sex:
|
Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS20: ventricle cardiac muscle |
Present |
Regionally restricted |
3H
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Expression assocated with cells invading ventricular myocardium.
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Specimen
4C:
embryonic day 11.5; Tnctm1Sia/Tnc +
(more )
Note: Double labeled: brown - Tnxb; blue - Tnc reporter (assay MGI:7645230).
Specimen
4C:
(close )
Genetic Background:
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involves: C57BL/6NCrlj * CBA/JNCrlj
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Mutant Allele(s):
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Tnctm1Sia/Tnc+
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
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Paraffin
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Note:
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Double labeled: brown - Tnxb; blue - Tnc reporter (assay MGI:7645230).
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: visceral pericardium |
Present |
Regionally restricted |
4C
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Expression in epicardial layer of diaphragm.
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Specimen
4D:
(close )
Genetic Background:
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involves: C57BL/6NCrlj * CBA/JNCrlj
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Mutant Allele(s):
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Tnctm1Sia/Tnctm1Sia
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
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Note:
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Double labeled: brown - Tnxb; blue - Tnc reporter (assay MGI:7645230).
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: visceral pericardium |
Present |
Regionally restricted |
4D
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No expression at sites of Tnc expression.
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