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Gene Expression Data
Assay Details
Assay
Reference: J:190449 Korostowski L, et al., The Kcnq1ot1 long non-coding RNA affects chromatin conformation and expression of Kcnq1, but does not regulate its imprinting in the developing heart. PLoS Genet. 2012 Sep;8(9):e1002956
Assay type: RT-PCR
MGI Accession ID: MGI:7647198
Gene symbol: Kcnq1
Gene name: potassium voltage-gated channel, subfamily Q, member 1
Probe: Kcnq1-pF, Kcnq1-pR AS-RT
Assay notes: Imprinting assay - allele-specific RT-PCR. Abbreviations: M, maternal; P, paternal; N, non-digested; D, Digested; C, B6(CAST7); KT, K-term.
Results
Image: 3A ©

 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified (a) Size Not Specified (b) Size Not Specified (c) Amount Genetic BackgroundMutant Allele(s)Sex Note
CxKT E10.5 Head N E10.5 TS17: head Not Applicable Present Not Applicable Not Specified; total RNA (C57BL/6J-Chr 7CAST/EiJ/Msb x 129S1/Sv-Kcnq1ot1tm3Tilg)F1 Kcnq1ot1tm3Tilg/Kcnq1ot1+Not Specified
CxKT E10.5 Head D E10.5 TS17: head Present Not Applicable Present Not Specified; total RNA (C57BL/6J-Chr 7CAST/EiJ/Msb x 129S1/Sv-Kcnq1ot1tm3Tilg)F1 Kcnq1ot1tm3Tilg/Kcnq1ot1+Not Specified Digested with NlaIII.
CxKT E10.5 Body N E10.5 TS17: trunk Not Applicable Present Not Applicable Not Specified; total RNA (C57BL/6J-Chr 7CAST/EiJ/Msb x 129S1/Sv-Kcnq1ot1tm3Tilg)F1 Kcnq1ot1tm3Tilg/Kcnq1ot1+Not Specified
CxKT E10.5 Body D E10.5 TS17: trunk Present Not Applicable Present Not Specified; total RNA (C57BL/6J-Chr 7CAST/EiJ/Msb x 129S1/Sv-Kcnq1ot1tm3Tilg)F1 Kcnq1ot1tm3Tilg/Kcnq1ot1+Not Specified Digested with NlaIII.
CxKT Heart E10.5 N E10.5 TS17: heart Not Applicable Present Not Applicable Not Specified; total RNA (C57BL/6J-Chr 7CAST/EiJ/Msb x 129S1/Sv-Kcnq1ot1tm3Tilg)F1 Kcnq1ot1tm3Tilg/Kcnq1ot1+Not Specified
CxKT Heart E10.5 D E10.5 TS17: heart Absent Not Applicable Present Not Specified; total RNA (C57BL/6J-Chr 7CAST/EiJ/Msb x 129S1/Sv-Kcnq1ot1tm3Tilg)F1 Kcnq1ot1tm3Tilg/Kcnq1ot1+Not Specified Digested with NlaIII.
CxKT Heart E11.5 N E11.5 TS19: heart Not Applicable Present Not Applicable Not Specified; total RNA (C57BL/6J-Chr 7CAST/EiJ/Msb x 129S1/Sv-Kcnq1ot1tm3Tilg)F1 Kcnq1ot1tm3Tilg/Kcnq1ot1+Not Specified
CxKT Heart E11.5 D E11.5 TS19: heart Absent Not Applicable Present Not Specified; total RNA (C57BL/6J-Chr 7CAST/EiJ/Msb x 129S1/Sv-Kcnq1ot1tm3Tilg)F1 Kcnq1ot1tm3Tilg/Kcnq1ot1+Not Specified Digested with NlaIII.
CxKT Heart E12.5 N E12.5 TS20: heart Not Applicable Present Not Applicable Not Specified; total RNA (C57BL/6J-Chr 7CAST/EiJ/Msb x 129S1/Sv-Kcnq1ot1tm3Tilg)F1 Kcnq1ot1tm3Tilg/Kcnq1ot1+Not Specified
CxKT Heart E12.5 D E12.5 TS20: heart Present Not Applicable Present Not Specified; total RNA (C57BL/6J-Chr 7CAST/EiJ/Msb x 129S1/Sv-Kcnq1ot1tm3Tilg)F1 Kcnq1ot1tm3Tilg/Kcnq1ot1+Not Specified Digested with NlaIII.
CxKT Heart E14.5 N E14.5 TS22: heart Not Applicable Present Not Applicable Not Specified; total RNA (C57BL/6J-Chr 7CAST/EiJ/Msb x 129S1/Sv-Kcnq1ot1tm3Tilg)F1 Kcnq1ot1tm3Tilg/Kcnq1ot1+Not Specified
CxKT Heart E14.5 D E14.5 TS22: heart Present Not Applicable Present Not Specified; total RNA (C57BL/6J-Chr 7CAST/EiJ/Msb x 129S1/Sv-Kcnq1ot1tm3Tilg)F1 Kcnq1ot1tm3Tilg/Kcnq1ot1+Not Specified Digested with NlaIII.
CxKT Heart E16.5 N E16.5 TS24: heart Not Applicable Present Not Applicable Not Specified; total RNA (C57BL/6J-Chr 7CAST/EiJ/Msb x 129S1/Sv-Kcnq1ot1tm3Tilg)F1 Kcnq1ot1tm3Tilg/Kcnq1ot1+Not Specified
CxKT Heart E16.5 D E16.5 TS24: heart Present Not Applicable Present Not Specified; total RNA (C57BL/6J-Chr 7CAST/EiJ/Msb x 129S1/Sv-Kcnq1ot1tm3Tilg)F1 Kcnq1ot1tm3Tilg/Kcnq1ot1+Not Specified Digested with NlaIII.
CxKT Heart P2 N P2 TS27: heart Not Applicable Present Not Applicable Not Specified; total RNA (C57BL/6J-Chr 7CAST/EiJ/Msb x 129S1/Sv-Kcnq1ot1tm3Tilg)F1 Kcnq1ot1tm3Tilg/Kcnq1ot1+Not Specified
CxKT Heart P2 D P2 TS27: heart Present Not Applicable Present Not Specified; total RNA (C57BL/6J-Chr 7CAST/EiJ/Msb x 129S1/Sv-Kcnq1ot1tm3Tilg)F1 Kcnq1ot1tm3Tilg/Kcnq1ot1+Not Specified Digested with NlaIII.
Notes:
(a) In the digested (D) lanes, this represents the KT band. Paternal.
(b) Non-digested (N) lanes.
(c) In the digested (D) lanes, this represents the B6(CAST7) band. Maternal.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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09/17/2024
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