About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:305426 Du G, et al., Proper timing of a quiescence period in precursor prospermatogonia is required for stem cell pool establishment in the male germline. Development. 2021 May 1;148(9):dev194571
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7662973
Gene symbol: Casp3
Gene name: caspase 3
Antibody: anti-cleaved Caspase-3 (Asp175; #9661)
Detection system: Secondary antibody
Results
Specimen 3F Control E14.5: embryonic day 14.5 (more )
Note: Double labeled: green - Casp3; red - Gcna (assay MGI:7662958).
Structure Level Pattern Image Note
TS22: germ cell of testis Present 3F Control E14.5
Specimen 3F Control E16.5: embryonic day 16.5 (more )
Note: Double labeled: green - Casp3; red - Gcna (assay MGI:7662958).
Structure Level Pattern Image Note
TS24: germ cell of testis Present Single cells 3F Control E16.5
Specimen 3F Control E18.5: embryonic day 18.5 (more )
Note: Double labeled: green - Casp3; red - Gcna (assay MGI:7662958).
Structure Level Pattern Image Note
TS26: germ cell of testis Present 3F Control E18.5
Specimen 3F Rb1-cKOBlimp1 E14.5: embryonic day 14.5; Rb1tm2Brn/Rb1tm2Brn, Tg(Prdm1-cre)1Masu/0 (more )
Note: Conditional mutant. Double labeled: green - Casp3; red - Gcna (assay MGI:7662958).
Structure Level Pattern Image Note
TS22: germ cell of testis Present 3F Rb1-cKOBlimp1 E14.5 The level of germ cell apoptosis was the same between control and this mutant.
Specimen 3F Rb1-cKOBlimp1 E16.5: embryonic day 16.5; Rb1tm2Brn/Rb1tm2Brn, Tg(Prdm1-cre)1Masu/0 (more )
Note: Conditional mutant. Double labeled: green - Casp3; red - Gcna (assay MGI:7662958).
Structure Level Pattern Image Note
TS24: germ cell of testis Present Single cells 3F Rb1-cKOBlimp1 E16.5 About 44.2% of prospermatogonia were apoptotic in this mutant compared with the 6.9% in control mice.
Specimen 3F Rb1-cKOBlimp1 E18.5: embryonic day 18.5; Rb1tm2Brn/Rb1tm2Brn, Tg(Prdm1-cre)1Masu/0 (more )
Note: Conditional mutant. Double labeled: green - Casp3; red - Gcna (assay MGI:7662958).
Structure Level Pattern Image Note
TS26: germ cell of testis Present 3F Rb1-cKOBlimp1 E18.5 The level of germ cell apoptosis in this mutant had increased compared to controls.
Specimen S2C Control: embryonic day 15.5 (more )
Note: Double labeled: green - Casp3; red - Gcna (assay MGI:7662958).
Structure Level Pattern Image Note
TS23: germ cell of testis Present S2C Control Cleaved CASPASE-3 , S2C Control Merge
Specimen S2C Rb1-cKOBlimp1: embryonic day 15.5; Rb1tm2Brn/Rb1tm2Brn, Tg(Prdm1-cre)1Masu/0 (more )
Note: Conditional mutant. Double labeled: green - Casp3; red - Gcna (assay MGI:7662958).
Structure Level Pattern Image Note
TS23: germ cell of testis Present S2C Rb1-cKOBlimp1 Merge , S2C Rb1-cKOBlimp1 Cleaved CASPASE-1 The level of germ cell apoptosis was the same between control and this mutant.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
10/09/2024
MGI 6.24 		The Jackson Laboratory