Gene Expression Data
Assay Details
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Assay
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Reference:
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J:336866
Puelles L, et al., The Midbrain Preisthmus: A Poorly Known Effect of the Isthmic Organizer. Int J Mol Sci. 2023 Jun 5;24(11)
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7664418
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Gene symbol:
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Fgf8
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Gene name:
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fibroblast growth factor 8
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Probe:
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Fgf8_riboprobe
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Assay notes:
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The authors analyzed data from the Allen Developing Mouse Brain.
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Results
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Specimen
2h:
embryonic day 11.5
(more  )
Note: Sample was postfixed in 4% paraformaldehyde.
Specimen
2h:
(close  )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Note:
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Sample was postfixed in 4% paraformaldehyde.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: midbrain-hindbrain junction |
Present |
Regionally restricted |
2h
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Signal was restricted at this stage to the rostral evaginated part of the isthmus, behind the IMB (isthmo-mesencephalic transverse boundary), at the caudal pole of the midbrain vesicle.
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Specimen
2i:
embryonic day 11.5
(more )
Note: Sample was postfixed in 4% paraformaldehyde.
Specimen
2i:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Note:
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Sample was postfixed in 4% paraformaldehyde.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: midbrain-hindbrain junction |
Present |
Regionally restricted |
2i
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Signal was restricted at this stage to the rostral evaginated part of the isthmus, behind the IMB (isthmo-mesencephalic transverse boundary), at the caudal pole of the midbrain vesicle.
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Specimen
4y:
embryonic day 13.5
(more )
Note: Sample was postfixed in 4% paraformaldehyde.
Specimen
4y:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Note:
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Sample was postfixed in 4% paraformaldehyde.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: midbrain-hindbrain junction |
Present |
Regionally restricted |
4y
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Dorsal and ventral intersections of the semicircular arch of Fgf8 expression along the rostralmost isthmus were detected.
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Specimen
9p:
embryonic day 18.5
(more )
Note: Sample was postfixed in 4% paraformaldehyde.
Specimen
9p:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 18.5
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Sex:
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Male
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Note:
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Sample was postfixed in 4% paraformaldehyde.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: midbrain-hindbrain junction |
Present |
Regionally restricted |
9p
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A remnant of Fgf8 activity was detected at a ventricular locus just caudally adjacent to the ventricular projection of the basal preisthmus.
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