Probe:	Ascl1_riboprobe
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase
Assay notes:	The authors analyzed data from the Allen Developing Mouse Brain.

Structure	Level	Pattern	Image	Note
TS19: midbrain ventricular layer	Present	Widespread	2n
TS19: midbrain	Present	Graded	2n	Expression showed a decreasing dorsocaudal gradient from the ventricular zone. Signal was sharply absent at a slightly evaginated part of the caudal midbrain neuroepithelium that covers the classic transverse sulcus intraencephalicus posterior.
TS19: midbrain mantle layer	Present	Regionally restricted	2n	Some expression was detected in the mantle ventrally.

Structure	Level	Pattern	Image	Note
TS19: midbrain ventricular layer	Present	Widespread	2o	Widespread expression was detected in the midbrain ventricular zone, except for weak expression in the caudal sulcal area ventricular zone.
TS19: midbrain	Present	Graded	2o	Expression showed a decreasing dorsocaudal gradient from the ventricular zone. Signal was sharply absent at a slightly evaginated part of the caudal midbrain neuroepithelium that covers the classic transverse sulcus intraencephalicus posterior.
TS19: midbrain mantle layer	Present	Regionally restricted	2o	Some expression was detected in the mantle ventrally.

Structure	Level	Pattern	Image	Note
TS22: midbrain mantle layer	Present		4k
TS22: superior colliculus	Present		4k
TS22: inferior colliculus	Weak		4k
TS22: midbrain	Present	Regionally restricted	4k	The preisthmic ventricular zone (including the sulcal area) was positive.
TS22: midbrain-hindbrain junction	Weak	Regionally restricted	4k	The isthmic ventricular zone showed a weak signal.

Structure	Level	Pattern	Image	Note
TS23: midbrain ventricular layer	Present	Regionally restricted	7c	Expression was detected in the ventricular zone of the preisthmus.
TS23: inferior colliculus	Absent		7c
TS23: midbrain-hindbrain junction	Absent		7c

Structure	Level	Pattern	Image	Note
TS23: midbrain ventricular layer	Present	Regionally restricted	7d	Expression was detected in the ventricular zone of the preisthmus and superior colliculus.
TS23: superior colliculus	Present	Regionally restricted	7d	Expression was detected in the ventricular zone of the preisthmus and superior colliculus.
TS23: inferior colliculus	Absent		7d
TS23: midbrain-hindbrain junction	Absent		7d