Gene Expression Data
Assay Details
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Assay
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Reference:
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J:336866
Puelles L, et al., The Midbrain Preisthmus: A Poorly Known Effect of the Isthmic Organizer. Int J Mol Sci. 2023 Jun 5;24(11)
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7664426
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Gene symbol:
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Gfra1
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Gene name:
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glial cell line derived neurotrophic factor family receptor alpha 1
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Probe:
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Gfra1_riboprobe
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Assay notes:
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The authors analyzed data from the Allen Mouse Brain and Developing Mouse Brain.
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Results
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Specimen
2p:
embryonic day 11.5
(more  )
Note: Sample was postfixed in 4% paraformaldehyde.
Specimen
2p:
(close  )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Note:
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Sample was postfixed in 4% paraformaldehyde.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: midbrain |
Ambiguous |
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2p
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A transverse negative gap apparently coinciding with the m2 locus and the associated sulcus at the caudal end of the midbrain vesicle was observed.
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Specimen
9m:
embryonic day 18.5
(more )
Note: Sample was postfixed in 4% paraformaldehyde.
Specimen
9m:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 18.5
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Sex:
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Male
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Note:
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Sample was postfixed in 4% paraformaldehyde.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: midbrain |
Present |
Regionally restricted |
9m
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Expression was detected in the preisthmus.
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| TS26: superior colliculus |
Present |
Regionally restricted |
9m
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Expression was detected in the superficial and intermediate strata.
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Specimen
9n:
embryonic day 18.5
(more )
Note: Sample was postfixed in 4% paraformaldehyde.
Specimen
9n:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 18.5
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Sex:
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Male
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Note:
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Sample was postfixed in 4% paraformaldehyde.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: midbrain |
Present |
Regionally restricted |
9n
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Expression was detected in the preisthmus. The rostral tectal gray visual formation was negative.
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| TS26: superior colliculus |
Present |
Regionally restricted |
9n
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Expression was detected in the superficial and intermediate strata.
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Specimen
9o:
embryonic day 18.5
(more )
Note: Sample was postfixed in 4% paraformaldehyde.
Specimen
9o:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 18.5
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Sex:
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Male
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Note:
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Sample was postfixed in 4% paraformaldehyde.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: midbrain |
Present |
Regionally restricted |
9o
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Expression was detected in the preisthmus. The rostral tectal gray visual formation was negative.
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| TS26: superior colliculus |
Present |
Regionally restricted |
9o
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Expression was detected in the superficial and intermediate strata.
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Specimen
9q:
embryonic day 18.5
(more )
Note: Sample was postfixed in 4% paraformaldehyde.
Specimen
9q:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 18.5
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Sex:
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Male
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Note:
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Sample was postfixed in 4% paraformaldehyde.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: midbrain |
Present |
Regionally restricted |
9q
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Expression was detected in the preisthmus. The rostral tectal gray visual formation was negative.
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| TS26: superior colliculus |
Present |
Regionally restricted |
9q
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Expression was detected in the superficial and intermediate strata.
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Specimen
9r:
embryonic day 18.5
(more )
Note: Sample was postfixed in 4% paraformaldehyde.
Specimen
9r:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 18.5
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Sex:
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Male
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Note:
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Sample was postfixed in 4% paraformaldehyde.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: midbrain |
Present |
Regionally restricted |
9r
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Expression was detected in the preisthmus. The rostral tectal gray visual formation was negative.
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| TS26: superior colliculus |
Present |
Regionally restricted |
9r
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Expression was detected in the superficial and intermediate strata.
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Specimen
9s:
embryonic day 18.5
(more )
Note: Sample was postfixed in 4% paraformaldehyde.
Specimen
9s:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 18.5
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Sex:
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Male
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Note:
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Sample was postfixed in 4% paraformaldehyde.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: midbrain |
Present |
Regionally restricted |
9s
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Expression was detected in the preisthmus. The rostral tectal gray visual formation was negative.
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| TS26: superior colliculus |
Present |
Regionally restricted |
9s
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Expression was detected in the superficial and intermediate strata.
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Specimen
12f:
postnatal day 56
(more )
Note: Sample was postfixed in 4% paraformaldehyde.
Specimen
12f:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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postnatal day 56
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Sex:
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Male
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Note:
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Sample was postfixed in 4% paraformaldehyde.
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