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Gene Expression Data
Assay Details
Assay
Reference: J:336866 Puelles L, et al., The Midbrain Preisthmus: A Poorly Known Effect of the Isthmic Organizer. Int J Mol Sci. 2023 Jun 5;24(11)
Assay type: RNA in situ
MGI Accession ID: MGI:7664428
Gene symbol: Id4
Gene name: inhibitor of DNA binding 4
Probe: Id4_riboprobe3
Probe preparation: Antisense labelled with digoxigenin RNA
Visualized with: Alkaline phosphatase
Assay notes: The authors analyzed data from the Allen Developing Mouse Brain.
Results
Specimen 2r: embryonic day 11.5 (more )
Note: Sample was postfixed in 4% paraformaldehyde.
Structure Level Pattern Image Note
TS19: midbrain Present Regionally restricted 2r Expression was detected in the rostral midbrain, but left a caudal midbrain gap devoid of signal, which may correspond to the prospective mesomere 2 (m2).
TS19: midbrain-hindbrain junction Present 2r
TS19: metencephalon Present Regionally restricted 2r Expression was detected in the rostral hindbrain.
Specimen 7s: embryonic day 15.5 (more )
Note: Sample was postfixed in 4% paraformaldehyde.
Structure Level Pattern Image Note
TS23: midbrain Present Regionally restricted 7s Expression was detected in the preisthmus and showed a curved boundary with the isthmus, with development of a marked convexity towards the midbrain.
Specimen 13h: postnatal day 4 (more )
Note: Sample was postfixed in 4% paraformaldehyde.
Structure Level Pattern Image Note
TS28: periaqueductal grey Present Regionally restricted 13h Selective labeling of the preisthmic patches of periaqueductal gray was observed.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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10/09/2024
MGI 6.24 		The Jackson Laboratory