Probe:	Lhx5_riboprobe2
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase
Assay notes:	The authors analyzed data from the Allen Developing Mouse Brain.

Structure	Level	Pattern	Image	Note
TS19: midbrain mantle layer	Present	Regionally restricted	2y	Expression was detected in the midbrain mantle at an intermediate rostrocaudal level, leaving negative both the rostral pole and the caudal sulcal area.
TS19: midbrain-hindbrain junction	Present		2y

Structure	Level	Pattern	Image	Note
TS19: midbrain mantle layer	Present	Regionally restricted	2z	Expression was detected in the midbrain mantle at an intermediate rostrocaudal level, leaving negative both the rostral pole and the caudal sulcal area.
TS19: midbrain-hindbrain junction	Present		2z

Structure	Level	Pattern	Image	Note
TS19: midbrain mantle layer	Present	Regionally restricted	2aa	Expression was detected in the midbrain mantle at an intermediate rostrocaudal level, leaving negative both the rostral pole and the caudal sulcal area.
TS19: midbrain-hindbrain junction	Present		2aa

Structure	Level	Pattern	Image	Note
TS22: superior colliculus	Present		4j
TS22: midbrain	Present	Regionally restricted	4j	Signal was detected in the preisthmic mantle domains (including the caudal sulcal area).
TS22: inferior colliculus	Ambiguous		4j	The inferior colliculus mantle and ventricular zones were largely negative.
TS22: midbrain-hindbrain junction	Absent		4j

Structure	Level	Pattern	Image	Note
TS22: superior colliculus	Present		6h
TS22: midbrain	Present	Regionally restricted	6h	Expression was detected in the preisthmus, including the caudal sulcal area.
TS22: inferior colliculus	Present	Regionally restricted	6h	A negative inferior colliculus domain was intercalated between the superior colliculus and the preisthmic domains. A subpial positive cell stream seems to invade the superficial inferior colliculus mantle out of the preisthmus.
TS22: midbrain-hindbrain junction	Present		6h

Structure	Level	Pattern	Image	Note
TS22: superior colliculus	Present		6i
TS22: midbrain	Present	Regionally restricted	6i	Expression was detected in the preisthmus, including the caudal sulcal area.
TS22: inferior colliculus	Present	Regionally restricted	6i	A negative inferior colliculus domain was intercalated between the superior colliculus and the preisthmic domains. A subpial positive cell stream seems to invade the superficial inferior colliculus mantle out of the preisthmus.
TS22: midbrain-hindbrain junction	Present		6i

Structure	Level	Pattern	Image	Note
TS22: superior colliculus	Present		6j
TS22: midbrain	Present	Regionally restricted	6j	Expression was detected in the preisthmus, including the caudal sulcal area.
TS22: inferior colliculus	Present	Regionally restricted	6j	A negative inferior colliculus domain was intercalated between the superior colliculus and the preisthmic domains. A subpial positive cell stream seems to invade the superficial inferior colliculus mantle out of the preisthmus.
TS22: midbrain-hindbrain junction	Present		6j

Structure	Level	Pattern	Image	Note
TS22: superior colliculus	Present		6k
TS22: midbrain	Present	Regionally restricted	6k	Expression was detected in the preisthmus, including the caudal sulcal area.
TS22: inferior colliculus	Present	Regionally restricted	6k	A negative inferior colliculus domain was intercalated between the superior colliculus and the preisthmic domains. A subpial positive cell stream seems to invade the superficial inferior colliculus mantle out of the preisthmus.
TS22: midbrain-hindbrain junction	Present		6k

Structure	Level	Pattern	Image	Note
TS22: superior colliculus	Present		6l
TS22: midbrain	Present	Regionally restricted	6l	Expression was detected in the preisthmus, including the caudal sulcal area.
TS22: inferior colliculus	Present	Regionally restricted	6l	A negative inferior colliculus domain was intercalated between the superior colliculus and the preisthmic domains. A subpial positive cell stream seems to invade the superficial inferior colliculus mantle out of the preisthmus.
TS22: midbrain-hindbrain junction	Present		6l

Structure	Level	Pattern	Image	Note
TS22: superior colliculus	Present		6m
TS22: midbrain	Present	Regionally restricted	6m	Expression was detected in the preisthmus, including the caudal sulcal area.
TS22: inferior colliculus	Present	Regionally restricted	6m	A negative inferior colliculus domain was intercalated between the superior colliculus and the preisthmic domains. A subpial positive cell stream seems to invade the superficial inferior colliculus mantle out of the preisthmus.
TS22: midbrain-hindbrain junction	Present		6m

Structure	Level	Pattern	Image	Note
TS22: superior colliculus	Present		6n
TS22: midbrain	Present	Regionally restricted	6n	Expression was detected in the preisthmus, including the caudal sulcal area.
TS22: inferior colliculus	Present	Regionally restricted	6n	A negative inferior colliculus domain was intercalated between the superior colliculus and the preisthmic domains. A subpial positive cell stream seems to invade the superficial inferior colliculus mantle out of the preisthmus.
TS22: midbrain-hindbrain junction	Present		6n

Structure	Level	Pattern	Image	Note
TS23: midbrain	Present	Regionally restricted	7f	Expression was detected in the preisthmus.

Structure	Level	Pattern	Image	Note
TS23: inferior colliculus	Absent		8q
TS23: superior colliculus	Weak	Diffuse	8q
TS23: midbrain	Present	Regionally restricted	8q	Expression was detected in the preisthmus mantle. The preisthmic domain dwindles in extent ventralwards.
TS23: midbrain-hindbrain junction	Absent		8q

Structure	Level	Pattern	Image	Note
TS23: inferior colliculus	Absent		8r
TS23: superior colliculus	Weak	Diffuse	8r
TS23: midbrain	Present	Regionally restricted	8r	Expression was detected in the preisthmus mantle. The preisthmic domain dwindles in extent ventralwards.
TS23: midbrain-hindbrain junction	Absent		8r

Structure	Level	Pattern	Image	Note
TS23: inferior colliculus	Absent		8s
TS23: superior colliculus	Weak	Diffuse	8s
TS23: midbrain	Present	Regionally restricted	8s	Expression was detected in the preisthmus mantle. The preisthmic domain dwindles in extent ventralwards.
TS23: midbrain-hindbrain junction	Absent		8s

Structure	Level	Pattern	Image	Note
TS23: inferior colliculus	Absent		8t
TS23: superior colliculus	Weak	Diffuse	8t
TS23: midbrain	Present	Regionally restricted	8t	Expression was detected in the preisthmus mantle. The preisthmic domain dwindles in extent ventralwards.
TS23: midbrain-hindbrain junction	Absent		8t