Probe:	Lhx2_riboprobe1
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase
Assay notes:	The authors analyzed data from the Allen Developing Mouse Brain.

Structure	Level	Pattern	Image	Note
TS19: midbrain	Present	Regionally restricted	2w	Label was detected in parts of prospective mesomere 1, but left out the caudal sulcal midbrain domain.

Structure	Level	Pattern	Image	Note
TS19: midbrain	Present	Regionally restricted	2x	Label was detected in parts of prospective mesomere 1, but left out the caudal sulcal midbrain domain.

Structure	Level	Pattern	Image	Note
TS22: midbrain mantle layer	Present	Regionally restricted	6a	Expression labeled deep mantle cells at the superior colliculus, inferior colliculus, and preisthmus.
TS22: superior colliculus	Present	Regionally restricted	6a	Expression labeled deep mantle cells.
TS22: inferior colliculus	Present	Regionally restricted	6a	Expression labeled deep mantle cells. A substantial superficial mass remained devoid of expression, particularly in the more ventral sections.

Structure	Level	Pattern	Image	Note
TS22: midbrain mantle layer	Present	Regionally restricted	6b	Expression labeled deep mantle cells at the superior colliculus, inferior colliculus, and preisthmus.
TS22: superior colliculus	Present	Regionally restricted	6b	Expression labeled deep mantle cells.
TS22: inferior colliculus	Present	Regionally restricted	6b	Expression labeled deep mantle cells. A substantial superficial mass remained devoid of expression, particularly in the more ventral sections.

Structure	Level	Pattern	Image	Note
TS22: midbrain mantle layer	Present	Regionally restricted	6c	Expression labeled deep mantle cells at the superior colliculus, inferior colliculus, and preisthmus.
TS22: superior colliculus	Present	Regionally restricted	6c	Expression labeled deep mantle cells.
TS22: inferior colliculus	Present	Regionally restricted	6c	Expression labeled deep mantle cells. A substantial superficial mass remained devoid of expression, particularly in the more ventral sections.

Structure	Level	Pattern	Image	Note
TS22: midbrain mantle layer	Present	Regionally restricted	6d	Expression labeled deep mantle cells at the superior colliculus, inferior colliculus, and preisthmus.
TS22: superior colliculus	Present	Regionally restricted	6d	Expression labeled deep mantle cells.
TS22: inferior colliculus	Present	Regionally restricted	6d	Expression labeled deep mantle cells. A substantial superficial mass remained devoid of expression, particularly in the more ventral sections.

Structure	Level	Pattern	Image	Note
TS22: midbrain mantle layer	Present	Regionally restricted	6e	Expression labeled deep mantle cells at the superior colliculus, inferior colliculus, and preisthmus.
TS22: superior colliculus	Present	Regionally restricted	6e	Expression labeled deep mantle cells.
TS22: inferior colliculus	Present	Regionally restricted	6e	Expression labeled deep mantle cells. A substantial superficial mass remained devoid of expression, particularly in the more ventral sections.

Structure	Level	Pattern	Image	Note
TS22: midbrain mantle layer	Present	Regionally restricted	6f	Expression labeled deep mantle cells at the superior colliculus, inferior colliculus, and preisthmus.
TS22: superior colliculus	Present	Regionally restricted	6f	Expression labeled deep mantle cells.
TS22: inferior colliculus	Present	Regionally restricted	6f	Expression labeled deep mantle cells. A substantial superficial mass remained devoid of expression, particularly in the more ventral sections.

Structure	Level	Pattern	Image	Note
TS23: midbrain	Present	Regionally restricted	8m	A negative inferior colliculus core nucleus was observed with an underlying positive periventricular stratum, which is continuous with the preisthmic mantle, as well as with the superior colliculus counterpart. The superficial mantle intercalated between the negative inferior colliculus and superior colliculus domains showed an increased signal of Lhx2.
TS23: inferior colliculus	Present	Regionally restricted	8m	A negative inferior colliculus core nucleus was observed with an underlying positive periventricular stratum.
TS23: superior colliculus	Present		8m

Structure	Level	Pattern	Image	Note
TS23: midbrain	Present	Regionally restricted	8n	A negative inferior colliculus core nucleus was observed with an underlying positive periventricular stratum, which is continuous with the preisthmic mantle, as well as with the superior colliculus counterpart. The superficial mantle intercalated between the negative inferior colliculus and superior colliculus domains showed an increased signal of Lhx2.
TS23: inferior colliculus	Present	Regionally restricted	8n	A negative inferior colliculus core nucleus was observed with an underlying positive periventricular stratum.
TS23: superior colliculus	Present		8n

Structure	Level	Pattern	Image	Note
TS23: midbrain	Present	Regionally restricted	8o	A negative inferior colliculus core nucleus was observed with an underlying positive periventricular stratum, which is continuous with the preisthmic mantle, as well as with the superior colliculus counterpart. The superficial mantle intercalated between the negative inferior colliculus and superior colliculus domains showed an increased signal of Lhx2.
TS23: inferior colliculus	Present	Regionally restricted	8o	A negative inferior colliculus core nucleus was observed with an underlying positive periventricular stratum.
TS23: superior colliculus	Present		8o

Structure	Level	Pattern	Image	Note
TS23: midbrain	Present	Regionally restricted	8p	A negative inferior colliculus core nucleus was observed with an underlying positive periventricular stratum, which is continuous with the preisthmic mantle, as well as with the superior colliculus counterpart. The superficial mantle intercalated between the negative inferior colliculus and superior colliculus domains showed an increased signal of Lhx2.
TS23: inferior colliculus	Present	Regionally restricted	8p	A negative inferior colliculus core nucleus was observed with an underlying positive periventricular stratum.
TS23: superior colliculus	Present		8p

Structure	Level	Pattern	Image	Note
TS26: midbrain	Present	Regionally restricted	9t	Signal illustrates the dorsomedial continuity of the positive preisthmic mantle with the symmetric toral bulges that have emerged out of the paramedian roof area, just in front of the isthmus. Lhx2 distinctly labels the ventricular zone and part of the mantle stratum of the toral bulges.

Structure	Level	Pattern	Image	Note
TS26: midbrain	Present	Regionally restricted	9u	Signal illustrates the dorsomedial continuity of the positive preisthmic mantle with the symmetric toral bulges that have emerged out of the paramedian roof area, just in front of the isthmus. Lhx2 distinctly labels the ventricular zone and part of the mantle stratum of the toral bulges.

Structure	Level	Pattern	Image	Note
TS26: midbrain	Present	Regionally restricted	9v	Signal illustrates the dorsomedial continuity of the positive preisthmic mantle with the symmetric toral bulges that have emerged out of the paramedian roof area, just in front of the isthmus. Lhx2 distinctly labels the ventricular zone and part of the mantle stratum of the toral bulges.

Structure	Level	Pattern	Image	Note
TS26: midbrain	Present	Regionally restricted	9w	Expression was detected in the preisthmus.