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Gene Expression Data
Assay Details
Assay
Reference: J:336866 Puelles L, et al., The Midbrain Preisthmus: A Poorly Known Effect of the Isthmic Organizer. Int J Mol Sci. 2023 Jun 5;24(11)
Assay type: RNA in situ
MGI Accession ID: MGI:7664436
Gene symbol: Sfrp1
Gene name: secreted frizzled-related protein 1
Probe: Sfrp1_riboprobe1
Probe preparation: Antisense labelled with digoxigenin RNA
Visualized with: Alkaline phosphatase
Assay notes: The authors analyzed data from the Allen Developing Mouse Brain.
Results
Specimen 2cc: embryonic day 11.5 (more )
Note: Sample was postfixed in 4% paraformaldehyde.
Structure Level Pattern Image Note
TS19: midbrain Present Regionally restricted 2cc Label was detected in parts of prospective mesomere 1, but left out the caudal sulcal midbrain domain.
Specimen 3e: embryonic day 11.5 (more )
Note: Sample was postfixed in 4% paraformaldehyde.
Structure Level Pattern Image Note
TS19: midbrain-hindbrain junction Strong 3e
TS19: midbrain Present Regionally restricted 3e The caudal sulcal midbrain area was not labeled.
Specimen 3f: embryonic day 11.5 (more )
Note: Sample was postfixed in 4% paraformaldehyde.
Structure Level Pattern Image Note
TS19: midbrain-hindbrain junction Strong 3f
TS19: midbrain Present Regionally restricted 3f The caudal sulcal midbrain area was not labeled.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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10/09/2024
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