Gene Expression Data
Assay Details
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Assay
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Reference:
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J:336866
Puelles L, et al., The Midbrain Preisthmus: A Poorly Known Effect of the Isthmic Organizer. Int J Mol Sci. 2023 Jun 5;24(11)
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7664439
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Gene symbol:
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Pax2
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Gene name:
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paired box 2
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Probe:
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Pax2_riboprobe
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Assay notes:
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The authors analyzed data from the Allen Developing Mouse Brain.
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Results
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Specimen
3g:
embryonic day 11.5
(more  )
Note: Sample was postfixed in 4% paraformaldehyde.
Specimen
3g:
(close  )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Note:
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Sample was postfixed in 4% paraformaldehyde.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: midbrain-hindbrain junction |
Weak |
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3g
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Expression stopped rostrally at the IMB (isthmo-mesencephalic transverse boundary), just caudal to the negative midbrain sulcal area.
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| TS19: midbrain |
Ambiguous |
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3g
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Mesomere 2 (m2) and the sulcal area were devoid of staining.
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Analysis Tools
