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Gene Expression Data
Assay Details
Assay
Reference: J:336866 Puelles L, et al., The Midbrain Preisthmus: A Poorly Known Effect of the Isthmic Organizer. Int J Mol Sci. 2023 Jun 5;24(11)
Assay type: RNA in situ
MGI Accession ID: MGI:7664440
Gene symbol: Pax8
Gene name: paired box 8
Probe: Pax8_riboprobe
Probe preparation: Antisense labelled with digoxigenin RNA
Visualized with: Alkaline phosphatase
Assay notes: The authors analyzed data from the Allen Developing Mouse Brain.
Results
Specimen 3h: embryonic day 11.5 (more )
Note: Sample was postfixed in 4% paraformaldehyde.
Structure Level Pattern Image Note
TS19: midbrain-hindbrain junction Weak 3h Expression stopped rostrally at the IMB (isthmo-mesencephalic transverse boundary), just caudal to the negative midbrain sulcal area.
TS19: midbrain Ambiguous 3h The sulcal area was devoid of staining.
Specimen 5h: embryonic day 13.5 (more )
Note: Sample was postfixed in 4% paraformaldehyde.
Structure Level Pattern Image Note
TS22: midbrain Present Regionally restricted 5h Expression was detected in the preisthmus.
Specimen 7aa: embryonic day 15.5 (more )
Note: Sample was postfixed in 4% paraformaldehyde.
Structure Level Pattern Image Note
TS23: midbrain Present Regionally restricted 7aa Expression in the superficial preisthmic mantle stratum was detected in a curved shape above the isthmic superficial elements with a ventral head-like thickening (oriented topographically rostral) and a longer dorsal tail portion (oriented topographically caudal).
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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10/09/2024
MGI 6.24 		The Jackson Laboratory