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Gene Expression Data
Assay Details
Assay
Reference: J:336866 Puelles L, et al., The Midbrain Preisthmus: A Poorly Known Effect of the Isthmic Organizer. Int J Mol Sci. 2023 Jun 5;24(11)
Assay type: RNA in situ
MGI Accession ID: MGI:7664441
Gene symbol: Pax5
Gene name: paired box 5
Probe: Pax5_riboprobe
Probe preparation: Antisense labelled with digoxigenin RNA
Visualized with: Alkaline phosphatase
Assay notes: The authors analyzed data from the Allen Developing Mouse Brain.
Results
Specimen 3i: embryonic day 11.5 (more )
Note: Sample was postfixed in 4% paraformaldehyde.
Structure Level Pattern Image Note
TS19: midbrain-hindbrain junction Present 3i
TS19: midbrain Present Regionally restricted 3i Expressed extended rostralward from the positive isthmus and penetrated the caudal part of the midbrain.
Specimen 3j: embryonic day 11.5 (more )
Note: Sample was postfixed in 4% paraformaldehyde.
Structure Level Pattern Image Note
TS19: midbrain-hindbrain junction Present 3j
TS19: midbrain Present Regionally restricted 3j Expressed extended rostralward from the positive isthmus and penetrated the caudal part of the midbrain.
Specimen 3k: embryonic day 11.5 (more )
Note: Sample was postfixed in 4% paraformaldehyde.
Structure Level Pattern Image Note
TS19: midbrain-hindbrain junction Present 3k
TS19: midbrain Present Regionally restricted 3k Expressed extended rostralward from the positive isthmus and penetrated the caudal part of the midbrain.
Specimen 4cc: embryonic day 13.5 (more )
Note: Sample was postfixed in 4% paraformaldehyde.
Structure Level Pattern Image Note
TS22: midbrain Present Regionally restricted 4cc Expression was detected in the preisthmus domain.
TS22: inferior colliculus Absent 4cc
TS22: midbrain-hindbrain junction Present 4cc
Specimen 4dd: embryonic day 13.5 (more )
Note: Sample was postfixed in 4% paraformaldehyde.
Structure Level Pattern Image Note
TS22: midbrain Present Regionally restricted 4dd Expression was detected in the preisthmus domain.
TS22: inferior colliculus Absent 4dd
TS22: midbrain-hindbrain junction Present 4dd
Specimen 4ee: embryonic day 13.5 (more )
Note: Sample was postfixed in 4% paraformaldehyde.
Structure Level Pattern Image Note
TS22: midbrain Present Regionally restricted 4ee Expression was detected in the preisthmus domain.
TS22: inferior colliculus Absent 4ee
TS22: midbrain-hindbrain junction Present 4ee
Specimen 5a: embryonic day 13.5 (more )
Note: Sample was postfixed in 4% paraformaldehyde.
Structure Level Pattern Image Note
TS22: midbrain Present Regionally restricted 5a Expression was detected in the preisthmus domain.
TS22: inferior colliculus Absent 5a
TS22: midbrain-hindbrain junction Present 5a
Specimen 5b: embryonic day 13.5 (more )
Note: Sample was postfixed in 4% paraformaldehyde.
Structure Level Pattern Image Note
TS22: midbrain Present Regionally restricted 5b Expression was detected in the preisthmus domain.
TS22: inferior colliculus Absent 5b
TS22: midbrain-hindbrain junction Present 5b
Specimen 5c: embryonic day 13.5 (more )
Note: Sample was postfixed in 4% paraformaldehyde.
Structure Level Pattern Image Note
TS22: midbrain Present Regionally restricted 5c Expression was detected in the preisthmus domain.
TS22: inferior colliculus Absent 5c
TS22: midbrain-hindbrain junction Present 5c
Specimen 5d: embryonic day 13.5 (more )
Note: Sample was postfixed in 4% paraformaldehyde.
Structure Level Pattern Image Note
TS22: midbrain Present Regionally restricted 5d Expression was detected in the preisthmus domain.
TS22: inferior colliculus Absent 5d
TS22: midbrain-hindbrain junction Present 5d
Specimen 5e: embryonic day 13.5 (more )
Note: Sample was postfixed in 4% paraformaldehyde.
Structure Level Pattern Image Note
TS22: midbrain Present Regionally restricted 5e Expression was detected in the preisthmus domain.
TS22: inferior colliculus Absent 5e
TS22: midbrain-hindbrain junction Present 5e
Specimen 5f: embryonic day 13.5 (more )
Note: Sample was postfixed in 4% paraformaldehyde.
Structure Level Pattern Image Note
TS22: midbrain Present Regionally restricted 5f Expression was detected in the preisthmus domain.
TS22: inferior colliculus Absent 5f
TS22: midbrain-hindbrain junction Present 5f
Specimen 5g: embryonic day 13.5 (more )
Note: Sample was postfixed in 4% paraformaldehyde.
Structure Level Pattern Image Note
TS22: midbrain Present Regionally restricted 5g Expression was detected in the preisthmus domain.
Specimen 7z: embryonic day 15.5 (more )
Note: Sample was postfixed in 4% paraformaldehyde.
Structure Level Pattern Image Note
TS23: midbrain Present Regionally restricted 7z Expression in the superficial preisthmic mantle stratum was detected in a curved shape above the isthmic superficial elements with a ventral head-like thickening (oriented topographically rostral) and a longer dorsal tail portion (oriented topographically caudal).
Specimen 10q: embryonic day 18.5 (more )
Note: Sample was postfixed in 4% paraformaldehyde.
Structure Level Pattern Image Note
TS26: midbrain Present Regionally restricted 10q Expression was detected in the superficial preisthmus.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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10/09/2024
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