Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:336866
Puelles L, et al., The Midbrain Preisthmus: A Poorly Known Effect of the Isthmic Organizer. Int J Mol Sci. 2023 Jun 5;24(11)
|
|
Assay type:
|
RNA in situ
|
|
MGI Accession ID:
|
MGI:7664443
|
|
Gene symbol:
|
Tal1
|
|
Gene name:
|
T cell acute lymphocytic leukemia 1
|
|
Probe:
|
Tal1_riboprobe
|
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
|
Visualized with:
|
Alkaline phosphatase
|
|
Assay notes:
|
The authors analyzed data from the Allen Developing Mouse Brain.
|
|
|
Results
|
Specimen
3m:
embryonic day 11.5
(more  )
Note: Sample was postfixed in 4% paraformaldehyde.
Specimen
3m:
(close  )
|
Genetic Background:
|
C57BL/6J
|
|
Age:
|
embryonic day 11.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Fresh Frozen
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Sample was postfixed in 4% paraformaldehyde.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS19: midbrain mantle layer |
Strong |
Regionally restricted |
3m
|
Strong expression was detected in the midbrain basal domain, but no staining was observed in the caudal sulcal area.
|
| TS19: midbrain ventricular layer |
Strong |
Regionally restricted |
3m
|
Strong expression was detected in the midbrain basal domain, but no staining was observed in the caudal sulcal area.
|
Specimen
5p:
embryonic day 13.5
(more )
Note: Sample was postfixed in 4% paraformaldehyde.
Specimen
5p:
(close )
|
Genetic Background:
|
C57BL/6J
|
|
Age:
|
embryonic day 13.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Fresh Frozen
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Sample was postfixed in 4% paraformaldehyde.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: midbrain |
Present |
Regionally restricted |
5p
|
Marked expression was detected laterally at the alar preisthmus.
|
Specimen
5q:
embryonic day 13.5
(more )
Note: Sample was postfixed in 4% paraformaldehyde.
Specimen
5q:
(close )
|
Genetic Background:
|
C57BL/6J
|
|
Age:
|
embryonic day 13.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Fresh Frozen
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Sample was postfixed in 4% paraformaldehyde.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: midbrain |
Present |
Regionally restricted |
5q
|
Expression was detected more ventrally along the transition into the preisthmic basal domain, where it is separated distinctly from an analogous isthmic basal signal by a negative narrow tissue gap associated with the posterior intraencephalic sulcus.
|
| TS22: midbrain-hindbrain junction |
Present |
Regionally restricted |
5q
|
A basal signal was detected in the isthmus.
|
Specimen
5r:
embryonic day 13.5
(more )
Note: Sample was postfixed in 4% paraformaldehyde.
Specimen
5r:
(close )
|
Genetic Background:
|
C57BL/6J
|
|
Age:
|
embryonic day 13.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Fresh Frozen
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Sample was postfixed in 4% paraformaldehyde.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: midbrain |
Present |
Regionally restricted |
5r
|
Expression was detected more ventrally along the transition into the preisthmic basal domain, where it is separated distinctly from an analogous isthmic basal signal by a negative narrow tissue gap associated with the posterior intraencephalic sulcus.
|
| TS22: midbrain-hindbrain junction |
Present |
Regionally restricted |
5r
|
A basal signal was detected in the isthmus.
|
Specimen
5s:
embryonic day 13.5
(more )
Note: Sample was postfixed in 4% paraformaldehyde.
Specimen
5s:
(close )
|
Genetic Background:
|
C57BL/6J
|
|
Age:
|
embryonic day 13.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Fresh Frozen
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Sample was postfixed in 4% paraformaldehyde.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: midbrain |
Present |
Regionally restricted |
5s
|
Expression was detected more ventrally along the transition into the preisthmic basal domain, where it is separated distinctly from an analogous isthmic basal signal by a negative narrow tissue gap associated with the posterior intraencephalic sulcus.
|
| TS22: midbrain-hindbrain junction |
Present |
Regionally restricted |
5s
|
A basal signal was detected in the isthmus.
|
Specimen
5t:
embryonic day 13.5
(more )
Note: Sample was postfixed in 4% paraformaldehyde.
Specimen
5t:
(close )
|
Genetic Background:
|
C57BL/6J
|
|
Age:
|
embryonic day 13.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Fresh Frozen
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Sample was postfixed in 4% paraformaldehyde.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: midbrain |
Present |
Regionally restricted |
5t
|
Expression was detected more ventrally along the transition into the preisthmic basal domain, where it is separated distinctly from an analogous isthmic basal signal by a negative narrow tissue gap associated with the posterior intraencephalic sulcus.
|
| TS22: midbrain-hindbrain junction |
Present |
Regionally restricted |
5t
|
A basal signal was detected in the isthmus.
|
Specimen
7t:
embryonic day 15.5
(more )
Note: Sample was postfixed in 4% paraformaldehyde.
Specimen
7t:
(close )
|
Genetic Background:
|
C57BL/6J
|
|
Age:
|
embryonic day 15.5
|
|
Sex:
|
Male
|
|
Type:
|
section
|
|
Fixation:
|
Fresh Frozen
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Sample was postfixed in 4% paraformaldehyde.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS23: midbrain |
Present |
Regionally restricted |
7t
|
Expression was detected in the preisthmus and showed a curved boundary with the isthmus, with development of a marked convexity towards the midbrain.
|
|
Analysis Tools