Probe:	Tal2_riboprobe
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase
Assay notes:	The authors analyzed data from the Allen Developing Mouse Brain.

Structure	Level	Pattern	Image	Note
TS19: midbrain mantle layer	Strong	Regionally restricted	3n	Strong expression was detected in the midbrain basal domain. Weak expression was also detected in the alar domain.
TS19: midbrain ventricular layer	Strong	Regionally restricted	3n	Strong expression was detected in the midbrain basal domain. Weak expression was also detected in the alar domain.
TS19: midbrain	Weak	Regionally restricted	3n	Weak expression was also detected in the alar domain. No staining was observed in the caudal sulcal area.

Structure	Level	Pattern	Image	Note
TS19: midbrain mantle layer	Strong	Regionally restricted	3o	Strong expression was detected in the midbrain basal domain. Weak expression was also detected in the alar domain.
TS19: midbrain ventricular layer	Strong	Regionally restricted	3o	Strong expression was detected in the midbrain basal domain. Weak expression was also detected in the alar domain.
TS19: midbrain	Weak	Regionally restricted	3o	Weak expression was also detected in the alar domain. No staining was observed in the caudal sulcal area.

Structure	Level	Pattern	Image	Note
TS19: midbrain mantle layer	Strong	Regionally restricted	3p	Strong expression was detected in the midbrain basal domain. Weak expression was also detected in the alar domain.
TS19: midbrain ventricular layer	Strong	Regionally restricted	3p	Strong expression was detected in the midbrain basal domain. Weak expression was also detected in the alar domain.
TS19: midbrain	Weak	Regionally restricted	3p	Weak expression was also detected in the alar domain. No staining was observed in the caudal sulcal area.

Structure	Level	Pattern	Image	Note
TS22: midbrain	Present	Regionally restricted	5u	Major labeling was observed at the preisthmus.
TS22: superior colliculus	Present		5u
TS22: inferior colliculus	Present	Regionally restricted	5u	A minor periventricular signal was detected in the inferior colliculus primordium.
TS22: midbrain-hindbrain junction	Weak	Regionally restricted	5u	There was only very weak ventricular expression at the isthmus.

Structure	Level	Pattern	Image	Note
TS22: midbrain	Present	Regionally restricted	5v	Major labeling was observed at the preisthmus.
TS22: superior colliculus	Present		5v
TS22: inferior colliculus	Present	Regionally restricted	5v	A minor periventricular signal was detected in the inferior colliculus primordium.
TS22: midbrain-hindbrain junction	Weak	Regionally restricted	5v	There was only very weak ventricular expression at the isthmus.

Structure	Level	Pattern	Image	Note
TS22: midbrain	Present	Regionally restricted	5w	Major labeling was observed at the preisthmus.
TS22: superior colliculus	Present		5w
TS22: inferior colliculus	Present	Regionally restricted	5w	A minor periventricular signal was detected in the inferior colliculus primordium.
TS22: midbrain-hindbrain junction	Weak	Regionally restricted	5w	There was only very weak ventricular expression at the isthmus.

Structure	Level	Pattern	Image	Note
TS22: midbrain	Present	Regionally restricted	5x	Major labeling was observed at the preisthmus.
TS22: superior colliculus	Present		5x
TS22: inferior colliculus	Present	Regionally restricted	5x	A minor periventricular signal was detected in the inferior colliculus primordium.
TS22: midbrain-hindbrain junction	Weak	Regionally restricted	5x	There was only very weak ventricular expression at the isthmus.

Structure	Level	Pattern	Image	Note
TS22: midbrain	Present	Regionally restricted	5y	Major labeling was observed at the preisthmus.
TS22: superior colliculus	Present		5y
TS22: inferior colliculus	Present	Regionally restricted	5y	A minor periventricular signal was detected in the inferior colliculus primordium.
TS22: midbrain-hindbrain junction	Weak	Regionally restricted	5y	There was only very weak ventricular expression at the isthmus.

Structure	Level	Pattern	Image	Note
TS22: midbrain	Present	Regionally restricted	5z	Major labeling was observed at the preisthmus.
TS22: superior colliculus	Present		5z
TS22: inferior colliculus	Present	Regionally restricted	5z	A minor periventricular signal was detected in the inferior colliculus primordium.
TS22: midbrain-hindbrain junction	Weak	Regionally restricted	5z	There was only very weak ventricular expression at the isthmus.

Structure	Level	Pattern	Image	Note
TS26: midbrain	Present	Regionally restricted	11i	Expression was detected in the ventricular zone of the toral bulges.
TS26: inferior colliculus	Absent		11i

Structure	Level	Pattern	Image	Note
TS26: midbrain	Present	Regionally restricted	11j	Expression was detected in the ventricular zone of the toral bulges. Signal was absent from the preisthmus.
TS26: inferior colliculus	Absent		11j

Structure	Level	Pattern	Image	Note
TS26: midbrain	Present	Regionally restricted	11k	Expression was detected in the ventricular zone of the toral bulges. Signal was absent from the preisthmus.
TS26: inferior colliculus	Absent		11k

Structure	Level	Pattern	Image	Note
TS26: midbrain	Present	Regionally restricted	11l	Expression was detected in the ventricular zone of the toral bulges. Signal was absent from the preisthmus.
TS26: inferior colliculus	Absent		11l