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Gene Expression Data
Assay Details
Assay
Reference: J:336866 Puelles L, et al., The Midbrain Preisthmus: A Poorly Known Effect of the Isthmic Organizer. Int J Mol Sci. 2023 Jun 5;24(11)
Assay type: RNA in situ
MGI Accession ID: MGI:7664445
Gene symbol: Sst
Gene name: somatostatin
Probe: Sst_riboprobe1
Probe preparation: Antisense labelled with digoxigenin RNA
Visualized with: Alkaline phosphatase
Assay notes: The authors analyzed data from the Allen Developing Mouse Brain.
Results
Specimen 3q: embryonic day 11.5 (more )
Note: Sample was postfixed in 4% paraformaldehyde.
Structure Level Pattern Image Note
TS19: midbrain Present Regionally restricted 3q Sst-positive postmitotic cells were observed rostrally in the basal midbrain but were lacking at the caudal sulcal area.
TS19: midbrain-hindbrain junction Present Regionally restricted 3q Sst-positive postmitotic cells were observed rostrally in the basal isthmus.

Specimen 5j: embryonic day 13.5 (more )
Note: Sample was postfixed in 4% paraformaldehyde.
Structure Level Pattern Image Note
TS22: midbrain Present Regionally restricted 5j Expression was detected in the preisthmus.

Specimen 8b: embryonic day 15.5 (more )
Note: Sample was postfixed in 4% paraformaldehyde.
Structure Level Pattern Image Note
TS23: inferior colliculus Present 8b Expression was detected in the inferior colliculus/brachium of the inferior colliculus complex.
TS23: midbrain-hindbrain junction Present Regionally restricted 8b Expression was detected in the isthmus mantle.

Specimen 8z: embryonic day 15.5 (more )
Note: Sample was postfixed in 4% paraformaldehyde.
Structure Level Pattern Image Note
TS23: midbrain Present Regionally restricted 8z Selective labeling was observed in the basal or tegmental preisthmus.

Specimen 11u: embryonic day 18.5 (more )
Note: Sample was postfixed in 4% paraformaldehyde.
Structure Level Pattern Image Note
TS26: midbrain Present Regionally restricted 11u Expression was detected in the basal tegmental preisthmus.

Specimen 12c: postnatal day 4 (more )
Note: Sample was postfixed in 4% paraformaldehyde.
Structure Level Pattern Image Note
TS28: midbrain Present Regionally restricted 12c Expression was detected in an elongated and narrow tissue band at the caudal end of the basal midbrain.


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
10/09/2024
MGI 6.24
The Jackson Laboratory