Gene Expression Data
Assay Details
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Assay
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Reference:
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J:336866
Puelles L, et al., The Midbrain Preisthmus: A Poorly Known Effect of the Isthmic Organizer. Int J Mol Sci. 2023 Jun 5;24(11)
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7664446
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Gene symbol:
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Trh
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Gene name:
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thyrotropin releasing hormone
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Probe:
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Trh_riboprobe
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Assay notes:
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The authors analyzed data from the Allen Developing Mouse Brain.
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Results
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Specimen
3r:
embryonic day 11.5
(more  )
Note: Sample was postfixed in 4% paraformaldehyde.
Specimen
3r:
(close  )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Note:
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Sample was postfixed in 4% paraformaldehyde.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: midbrain |
Present |
Regionally restricted |
3r
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Signal appeared selectively in a semilunar postmitotic mantle area that might correspond to the caudal sulcal area.
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Specimen
11z:
embryonic day 18.5
(more )
Note: Sample was postfixed in 4% paraformaldehyde.
Specimen
11z:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 18.5
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Sex:
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Male
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Note:
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Sample was postfixed in 4% paraformaldehyde.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: midbrain |
Present |
Regionally restricted |
11z
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A singular basal preisthmic dot-like area of expression was seen periventricularly.
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Specimen
11aa:
embryonic day 18.5
(more )
Note: Sample was postfixed in 4% paraformaldehyde.
Specimen
11aa:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 18.5
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Sex:
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Male
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Note:
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Sample was postfixed in 4% paraformaldehyde.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: midbrain |
Present |
Regionally restricted |
11aa
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A Trh-positive distinct cell group in the ventral periaqueductal gray associated with the preisthmus, out of which less numerous similar cells spread out laterally and ventrally.
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| TS26: periaqueductal grey |
Present |
Regionally restricted |
11aa
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A Trh-positive distinct cell group in the ventral periaqueductal gray associated with the preisthmus, out of which less numerous similar cells spread out laterally and ventrally.
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Analysis Tools