Probe:	Tcf7l2_riboprobe
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase
Assay notes:	The authors analyzed data from the Allen Developing Mouse Brain.

Structure	Level	Pattern	Image	Note
TS19: midbrain	Weak		3v
TS19: midbrain-hindbrain junction	Weak		3v

Structure	Level	Pattern	Image	Note
TS20: midbrain mantle layer	Present	Regionally restricted	3w	Positive mantle cells started to aggregate subpially, particularly in an intermediate domain of the midbrain lying rostral to the sulcal area.

Structure	Level	Pattern	Image	Note
TS22: midbrain	Present	Regionally restricted	5aa	Expression was detected in the superior colliculus and inferior colliculus mantle primordia, leaving negative the preisthmus and the caudal sulcal area.
TS22: inferior colliculus	Present	Regionally restricted	5aa	Expression was detected in the superior colliculus and inferior colliculus mantle primordia.
TS22: superior colliculus	Present	Regionally restricted	5aa	Expression was detected in the superior colliculus and inferior colliculus mantle primordia.
TS22: midbrain-hindbrain junction	Absent		5aa

Structure	Level	Pattern	Image	Note
TS22: midbrain	Present	Regionally restricted	5bb	Expression was detected in the superior colliculus and inferior colliculus mantle primordia, leaving negative the preisthmus and the caudal sulcal area.
TS22: inferior colliculus	Present	Regionally restricted	5bb	Expression was detected in the superior colliculus and inferior colliculus mantle primordia.
TS22: superior colliculus	Present	Regionally restricted	5bb	Expression was detected in the superior colliculus and inferior colliculus mantle primordia.
TS22: midbrain-hindbrain junction	Absent		5bb

Structure	Level	Pattern	Image	Note
TS22: midbrain	Present	Regionally restricted	5cc	Expression was detected in the superior colliculus and inferior colliculus mantle primordia, leaving negative the preisthmus and the caudal sulcal area.
TS22: inferior colliculus	Present	Regionally restricted	5cc	Expression was detected in the superior colliculus and inferior colliculus mantle primordia.
TS22: superior colliculus	Present	Regionally restricted	5cc	Expression was detected in the superior colliculus and inferior colliculus mantle primordia.
TS22: midbrain-hindbrain junction	Absent		5cc

Structure	Level	Pattern	Image	Note
TS22: midbrain	Present	Regionally restricted	5dd	Expression was detected in the superior colliculus and inferior colliculus mantle primordia, leaving negative the preisthmus and the caudal sulcal area.
TS22: inferior colliculus	Present	Regionally restricted	5dd	Expression was detected in the superior colliculus and inferior colliculus mantle primordia.
TS22: superior colliculus	Present	Regionally restricted	5dd	Expression was detected in the superior colliculus and inferior colliculus mantle primordia.
TS22: midbrain-hindbrain junction	Absent		5dd

Structure	Level	Pattern	Image	Note
TS22: midbrain	Present	Regionally restricted	5ee	Expression was detected in the superior colliculus and inferior colliculus mantle primordia, leaving negative the preisthmus and the caudal sulcal area.
TS22: inferior colliculus	Present	Regionally restricted	5ee	Expression was detected in the superior colliculus and inferior colliculus mantle primordia.
TS22: superior colliculus	Present	Regionally restricted	5ee	Expression was detected in the superior colliculus and inferior colliculus mantle primordia.
TS22: midbrain-hindbrain junction	Absent		5ee

Structure	Level	Pattern	Image	Note
TS22: midbrain	Present	Regionally restricted	5ff	Expression was detected in the superior colliculus and inferior colliculus mantle primordia, leaving negative the preisthmus and the caudal sulcal area.
TS22: inferior colliculus	Present	Regionally restricted	5ff	Expression was detected in the superior colliculus and inferior colliculus mantle primordia.
TS22: superior colliculus	Present	Regionally restricted	5ff	Expression was detected in the superior colliculus and inferior colliculus mantle primordia.
TS22: midbrain-hindbrain junction	Absent		5ff

Structure	Level	Pattern	Image	Note
TS23: inferior colliculus	Present	Regionally restricted	8i	Labeling occupies the whole mantle.
TS23: superior colliculus	Present		8i
TS23: midbrain	Present	Single cells	8i	The preisthmus was essentially negative except for a few periventricular cells.

Structure	Level	Pattern	Image	Note
TS23: inferior colliculus	Present	Regionally restricted	8j	Labeling occupies the whole mantle, while more rostrally, possibly at levels through the brachium of the inferior colliculus nucleus, it appears separated by a negative periventricular stratum from the corresponding positive ventricular zone.
TS23: superior colliculus	Present		8j
TS23: midbrain	Present	Single cells	8j	The preisthmus was essentially negative except for a few periventricular cells.

Structure	Level	Pattern	Image	Note
TS23: inferior colliculus	Present	Regionally restricted	8k	Labeling occupies the whole mantle, while more rostrally, possibly at levels through the brachium of the inferior colliculus nucleus, it appears separated by a negative periventricular stratum from the corresponding positive ventricular zone.
TS23: superior colliculus	Present		8k
TS23: midbrain	Present	Single cells	8k	The preisthmus was essentially negative except for a few periventricular cells.

Structure	Level	Pattern	Image	Note
TS23: inferior colliculus	Present	Regionally restricted	8l	Labeling occupies the whole mantle, while more rostrally, possibly at levels through the brachium of the inferior colliculus nucleus, it appears separated by a negative periventricular stratum from the corresponding positive ventricular zone.
TS23: superior colliculus	Present		8l
TS23: midbrain	Present	Single cells	8l	The preisthmus was essentially negative except for a few periventricular cells.

Structure	Level	Pattern	Image	Note
TS24: inferior colliculus	Present		11m
TS24: midbrain	Present	Regionally restricted	11m	Continuous expression was detected from the core of the inferior colliculus into the immature dorsomedial toral area, leaving the roof plate negative.

Structure	Level	Pattern	Image	Note
TS24: inferior colliculus	Present		11n
TS24: midbrain	Present	Regionally restricted	11n	Continuous expression was detected from the core of the inferior colliculus into the immature dorsomedial toral area, leaving the roof plate negative.

Structure	Level	Pattern	Image	Note
TS24: inferior colliculus	Present		11o
TS24: midbrain	Present	Regionally restricted	11o	The preisthmus appears negative, but a remnant of the positive toral area was present.

Structure	Level	Pattern	Image	Note
TS24: inferior colliculus	Present		11p
TS24: midbrain	Present	Regionally restricted	11p	The preisthmus appears negative, but a remnant of the positive toral area was present.

Structure	Level	Pattern	Image	Note
TS25: inferior colliculus	Present		11q
TS25: midbrain	Present	Regionally restricted	11q	Expression pattern was similar to E16.5.

Structure	Level	Pattern	Image	Note
TS25: inferior colliculus	Present		11r
TS25: midbrain	Present	Regionally restricted	11r	Expression pattern was similar to E16.5.

Structure	Level	Pattern	Image	Note
TS25: inferior colliculus	Present		11s
TS25: midbrain	Present	Regionally restricted	11s	Expression pattern was similar to E16.5.

Structure	Level	Pattern	Image	Note
TS26: inferior colliculus	Present		11v
TS26: midbrain	Present	Regionally restricted	11v	Expression pattern was similar to E16.5.

Structure	Level	Pattern	Image	Note
TS26: inferior colliculus	Present		11w
TS26: midbrain	Present	Regionally restricted	11w	Expression pattern was similar to E16.5.

Structure	Level	Pattern	Image	Note
TS26: inferior colliculus	Present		11x
TS26: midbrain	Present	Regionally restricted	11x	Expression pattern was similar to E16.5.

Structure	Level	Pattern	Image	Note
TS26: inferior colliculus	Present		11y
TS26: midbrain	Present	Regionally restricted	11y	Expression pattern was similar to E16.5.

Structure	Level	Pattern	Image	Note
TS27: inferior colliculus	Present		13d
TS27: midbrain	Present	Regionally restricted	13d	Expression was detected in the toral region.

Structure	Level	Pattern	Image	Note
TS27: inferior colliculus	Present		13e
TS27: midbrain	Present	Regionally restricted	13e	Expression was detected in the toral region.

Structure	Level	Pattern	Image	Note
TS28: inferior colliculus	Present		13f
TS28: midbrain	Present	Regionally restricted	13f	Expression was detected in the toral region.

Structure	Level	Pattern	Image	Note
TS28: midbrain	Present	Regionally restricted	13j	Expression was detected in the former toral bulges, which show marked flattening.