Probe:	Meis2_riboprobe2
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase
Assay notes:	The authors analyzed data from the Allen Developing Mouse Brain.

Structure	Level	Pattern	Image	Note
TS22: hindbrain	Present		4u
TS22: midbrain-hindbrain junction	Present		4u
TS22: superior colliculus	Weak		4u
TS22: inferior colliculus	Weak		4u	Signal was weak in the inferior colliculus and brachium of the inferior colliculus.
TS22: midbrain	Present	Regionally restricted	4u	Expression was detected in a basal patch of mantle cells in mesomere 1. The preisthmus was negative.

Structure	Level	Pattern	Image	Note
TS22: hindbrain	Present		4v
TS22: midbrain-hindbrain junction	Present		4v
TS22: superior colliculus	Weak		4v
TS22: inferior colliculus	Weak		4v	Signal was weak in the inferior colliculus and brachium of the inferior colliculus.
TS22: midbrain	Present	Regionally restricted	4v	Expression was detected in a basal patch of mantle cells in mesomere 1. The preisthmus was negative.

Structure	Level	Pattern	Image	Note
TS23: midbrain	Present	Regionally restricted	7u	Expression was negative in the preisthmus and most of the superior colliculus, but positive in the inferior colliculus/brachium of the inferior colliculus complex. There was strong labeling in the intercollicular domain (ICol), intercalated between the superior colliculus and the inferior colliculus.
TS23: inferior colliculus	Present	Regionally restricted	7u	Expression was detected in the inferior colliculus/brachium of the inferior colliculus complex.
TS23: midbrain-hindbrain junction	Strong		7u
TS23: metencephalon	Strong	Regionally restricted	7u	The hindbrain prepontine area was strongly positive.

Structure	Level	Pattern	Image	Note
TS23: midbrain	Present	Regionally restricted	7v	Expression was negative in the preisthmus and most of the superior colliculus, but positive in the inferior colliculus/brachium of the inferior colliculus complex. There was strong labeling in the intercollicular domain (ICol), intercalated between the superior colliculus and the inferior colliculus.
TS23: inferior colliculus	Present	Regionally restricted	7v	Expression was detected in the inferior colliculus/brachium of the inferior colliculus complex.
TS23: midbrain-hindbrain junction	Strong		7v
TS23: metencephalon	Strong	Regionally restricted	7v	The hindbrain prepontine area was strongly positive.

Structure	Level	Pattern	Image	Note
TS23: midbrain	Present	Regionally restricted	7w	Expression was negative in the preisthmus and most of the superior colliculus, but positive in the inferior colliculus/brachium of the inferior colliculus complex. There was strong labeling in the intercollicular domain (ICol), intercalated between the superior colliculus and the inferior colliculus. There was also a separate patch of Meis2 expression in the basal mesomere 1 area.
TS23: inferior colliculus	Present	Regionally restricted	7w	Expression was detected in the inferior colliculus/brachium of the inferior colliculus complex. The dorsal rim of the inferior colliculus domain shows a stronger signal than its ventral rim.
TS23: midbrain-hindbrain junction	Strong		7w
TS23: metencephalon	Strong	Regionally restricted	7w	The hindbrain prepontine area was strongly positive.

Structure	Level	Pattern	Image	Note
TS23: midbrain	Present	Regionally restricted	7x	Expression was negative in the preisthmus and most of the superior colliculus, but positive in the inferior colliculus/brachium of the inferior colliculus complex. There was strong labeling in the intercollicular domain (ICol), intercalated between the superior colliculus and the inferior colliculus. There was also a separate patch of Meis2 expression in the basal mesomere 1 area.
TS23: inferior colliculus	Present	Regionally restricted	7x	Expression was detected in the inferior colliculus/brachium of the inferior colliculus complex.
TS23: midbrain-hindbrain junction	Strong		7x
TS23: metencephalon	Strong	Regionally restricted	7x	The hindbrain prepontine area was strongly positive.

Structure	Level	Pattern	Image	Note
TS26: midbrain-hindbrain junction	Present	Regionally restricted	10j	Expression was detected in the subpial parabigeminal isthmic nucleus.

Structure	Level	Pattern	Image	Note
TS26: midbrain-hindbrain junction	Present		10k
TS26: inferior colliculus	Present		10k
TS26: midbrain	Present	Regionally restricted	10k	Higher Meis2 signal was detected in the intercollicular domain contrasting with the inferior colliculus/brachium of the inferior colliculus complex.

Structure	Level	Pattern	Image	Note
TS26: midbrain-hindbrain junction	Present		10l
TS26: inferior colliculus	Present		10l
TS26: midbrain	Present	Regionally restricted	10l	Higher Meis2 signal was detected in the intercollicular domain contrasting with the inferior colliculus/brachium of the inferior colliculus complex.

Structure	Level	Pattern	Image	Note
TS26: midbrain-hindbrain junction	Present		10m
TS26: inferior colliculus	Present		10m
TS26: midbrain	Present	Regionally restricted	10m	Higher Meis2 signal was detected in the intercollicular domain contrasting with the inferior colliculus/brachium of the inferior colliculus complex.

Structure	Level	Pattern	Image	Note
TS26: midbrain-hindbrain junction	Present		10n
TS26: inferior colliculus	Present		10n
TS26: midbrain	Present	Regionally restricted	10n	Higher Meis2 signal was detected in the intercollicular domain contrasting with the inferior colliculus/brachium of the inferior colliculus complex.

Structure	Level	Pattern	Image	Note
TS26: inferior colliculus	Present		10o
TS26: midbrain	Present	Regionally restricted	10o	Higher Meis2 signal was detected in the intercollicular domain contrasting with the inferior colliculus/brachium of the inferior colliculus complex.

Structure	Level	Pattern	Image	Note
TS26: midbrain	Present	Regionally restricted	10p	Strong expression was detected in the toral bulge.