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Gene Expression Data
Assay Details
Assay
Reference: J:336866 Puelles L, et al., The Midbrain Preisthmus: A Poorly Known Effect of the Isthmic Organizer. Int J Mol Sci. 2023 Jun 5;24(11)
Assay type: RNA in situ
MGI Accession ID: MGI:7664560
Gene symbol: Six3
Gene name: sine oculis-related homeobox 3
Probe: Six3_riboprobe
Probe preparation: Antisense labelled with digoxigenin RNA
Visualized with: Alkaline phosphatase
Assay notes: The authors analyzed data from the Allen Developing Mouse Brain.
Results
Specimen 5m: embryonic day 13.5 (more )
Note: Sample was postfixed in 4% paraformaldehyde.
Structure Level Pattern Image Note
TS22: midbrain Present Regionally restricted 5m Expression was detected in the preisthmus.
Specimen 5n: embryonic day 13.5 (more )
Note: Sample was postfixed in 4% paraformaldehyde.
Structure Level Pattern Image Note
TS22: midbrain Present Regionally restricted 5n Expression was detected in the preisthmus.
Specimen 8u: embryonic day 15.5 (more )
Note: Sample was postfixed in 4% paraformaldehyde.
Structure Level Pattern Image Note
TS23: inferior colliculus Present Regionally restricted 8u Positive cells were distributed through the lateral parts of the inferior colliculus/brachium of the inferior colliculus complex.
TS23: midbrain Strong Regionally restricted 8u Expression was strongly represented along the preisthmus.
TS23: superior colliculus Absent 8u
TS23: midbrain-hindbrain junction Absent 8u
Specimen 8v: embryonic day 15.5 (more )
Note: Sample was postfixed in 4% paraformaldehyde.
Structure Level Pattern Image Note
TS23: inferior colliculus Present Regionally restricted 8v Positive cells were distributed through the lateral parts of the inferior colliculus/brachium of the inferior colliculus complex.
TS23: midbrain Strong Regionally restricted 8v Expression was strongly represented along the preisthmus.
TS23: midbrain-hindbrain junction Absent 8v
TS23: superior colliculus Absent 8v
Specimen 8w: embryonic day 15.5 (more )
Note: Sample was postfixed in 4% paraformaldehyde.
Structure Level Pattern Image Note
TS23: inferior colliculus Present Regionally restricted 8w Positive cells were distributed through the lateral parts of the inferior colliculus/brachium of the inferior colliculus complex.
TS23: midbrain Strong Regionally restricted 8w Expression was strongly represented along the preisthmus.
TS23: midbrain-hindbrain junction Absent 8w
TS23: superior colliculus Absent 8w
Specimen 8x: embryonic day 15.5 (more )
Note: Sample was postfixed in 4% paraformaldehyde.
Structure Level Pattern Image Note
TS23: inferior colliculus Present Regionally restricted 8x Positive cells were distributed through the lateral parts of the inferior colliculus/brachium of the inferior colliculus complex.
TS23: midbrain Strong Regionally restricted 8x Expression was strongly represented along the preisthmus.
TS23: midbrain-hindbrain junction Absent 8x
TS23: superior colliculus Absent 8x
Specimen 13m: postnatal day 4 (more )
Note: Sample was postfixed in 4% paraformaldehyde.
Structure Level Pattern Image Note
TS28: midbrain Present Regionally restricted 13m Expression was detected in the former toral bulges, which show marked flattening.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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10/09/2024
MGI 6.24 		The Jackson Laboratory