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Gene Expression Data
Assay Details
Assay
Reference: J:336866 Puelles L, et al., The Midbrain Preisthmus: A Poorly Known Effect of the Isthmic Organizer. Int J Mol Sci. 2023 Jun 5;24(11)
Assay type: RNA in situ
MGI Accession ID: MGI:7664561
Gene symbol: Pou6f2
Gene name: POU domain, class 6, transcription factor 2
Probe: Pou6f2_riboprobe
Probe preparation: Antisense labelled with digoxigenin RNA
Visualized with: Alkaline phosphatase
Assay notes: The authors analyzed data from the Allen Developing Mouse Brain.
Results
Specimen 5k: embryonic day 13.5 (more )
Note: Sample was postfixed in 4% paraformaldehyde.
Structure Level Pattern Image Note
TS22: midbrain Present Regionally restricted 5k Expression was detected in the preisthmus.

Specimen 7bb: embryonic day 15.5 (more )
Note: Sample was postfixed in 4% paraformaldehyde.
Structure Level Pattern Image Note
TS23: midbrain Present Regionally restricted 7bb Expression in the superficial preisthmic mantle stratum was detected in a curved shape above the isthmic superficial elements with a ventral head-like thickening (oriented topographically rostral) and a longer dorsal tail portion (oriented topographically caudal).

Specimen 8aa: embryonic day 15.5 (more )
Note: Sample was postfixed in 4% paraformaldehyde.
Structure Level Pattern Image Note
TS23: midbrain Present Regionally restricted 8aa Selective labeling was observed in the basal or tegmental preisthmus.


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
10/09/2024
MGI 6.24
The Jackson Laboratory