Gene Expression Data
Assay Details
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Assay
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Reference:
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J:336866
Puelles L, et al., The Midbrain Preisthmus: A Poorly Known Effect of the Isthmic Organizer. Int J Mol Sci. 2023 Jun 5;24(11)
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7664569
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Gene symbol:
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Pou4f1
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Gene name:
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POU domain, class 4, transcription factor 1
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Probe:
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Pou4f1_riboprobe
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Assay notes:
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The authors analyzed data from the Allen Developing Mouse Brain.
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Results
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Specimen
6x:
embryonic day 13.5
(more  )
Note: Sample was postfixed in 4% paraformaldehyde.
Specimen
6x:
(close  )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Note:
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Sample was postfixed in 4% paraformaldehyde.
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS22: inferior colliculus |
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Present |
Regionally restricted |
6x
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Expression was detected in the mantle region in mesomere 1 (not the preisthmus in mesomere 2).
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| TS22: superior colliculus |
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Present |
Regionally restricted |
6x
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Expression was detected in the mantle region in mesomere 1 (not the preisthmus in mesomere 2).
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| TS22: brain nucleus |
neuron |
Present |
Regionally restricted |
6x
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Some positive mesencephalic nucleus of the trigeminal nerve (mes5) neurons remain fixed along the migration pathway targeting rhombomere 1.
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Specimen
6y:
embryonic day 13.5
(more )
Note: Sample was postfixed in 4% paraformaldehyde.
Specimen
6y:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Note:
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Sample was postfixed in 4% paraformaldehyde.
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS22: inferior colliculus |
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Present |
Regionally restricted |
6y
|
Expression was detected in the mantle region in mesomere 1 (not the preisthmus in mesomere 2).
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| TS22: superior colliculus |
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Present |
Regionally restricted |
6y
|
Expression was detected in the mantle region in mesomere 1 (not the preisthmus in mesomere 2).
|
| TS22: brain nucleus |
neuron |
Present |
Regionally restricted |
6y
|
Some positive mesencephalic nucleus of the trigeminal nerve (mes5) neurons remain fixed along the migration pathway targeting rhombomere 1.
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Analysis Tools