Probe:	Foxp2_riboprobe
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase
Assay notes:	The authors analyzed data from the Allen Developing Mouse Brain.

Structure	Level	Pattern	Image	Note
TS23: inferior colliculus	Present	Graded	8e	A deep to superficial gradiental labeling of the inferior colliculus primordium was observed.
TS23: superior colliculus	Weak		8e
TS23: midbrain	Weak	Regionally restricted	8e	Weak signal was detected at the intermediate stratum of the preisthmic mantle, caudal to the inferior colliculus.

Structure	Level	Pattern	Image	Note
TS23: inferior colliculus	Present	Graded	8f	A deep to superficial gradiental labeling of the inferior colliculus primordium was observed.
TS23: superior colliculus	Weak		8f
TS23: midbrain	Weak	Regionally restricted	8f	Weak signal was detected at the intermediate stratum of the preisthmic mantle, caudal to the inferior colliculus.

Structure	Level	Pattern	Image	Note
TS23: inferior colliculus	Present	Graded	8g	A deep to superficial gradiental labeling of the inferior colliculus primordium was observed.
TS23: superior colliculus	Weak		8g
TS23: midbrain	Weak	Regionally restricted	8g	Weak signal was detected at the intermediate stratum of the preisthmic mantle, caudal to the inferior colliculus.

Structure	Level	Pattern	Image	Note
TS23: inferior colliculus	Present	Graded	8h	A deep to superficial gradiental labeling of the inferior colliculus primordium was observed.
TS23: superior colliculus	Weak		8h
TS23: midbrain	Weak	Regionally restricted	8h	Weak signal was detected at the intermediate stratum of the preisthmic mantle, caudal to the inferior colliculus.
TS23: midbrain-hindbrain junction	Present	Regionally restricted	8h	The positive patch at the left bottom corner of the figure is an unidentified superficial isthmic mass.

Structure	Level	Pattern	Image	Note
TS26: inferior colliculus	Strong		10a	A series of eight dorsoventral horizontal sections show that the inferior colliculus core extends in a hook pattern, passing above the preisthmus and into the dorsomedial toral area next to the roof plate.
TS26: superior colliculus	Present	Regionally restricted	10a	Expression was detected in multiple layers in the superior colliculus.
TS26: midbrain	Strong	Regionally restricted	10a	Signal was strong in the inferior colliculus. Signal was detected in the superior colliculus and the mantle of the toral bulges. Signal was not detected in the intercollicular area.

Structure	Level	Pattern	Image	Note
TS26: inferior colliculus	Strong		10b	A series of eight dorsoventral horizontal sections show that the inferior colliculus core extends in a hook pattern, passing above the preisthmus and into the dorsomedial toral area next to the roof plate.
TS26: superior colliculus	Present	Regionally restricted	10b	Expression was detected in multiple layers in the superior colliculus.
TS26: midbrain	Strong	Regionally restricted	10b	Signal was strong in the inferior colliculus. Signal was detected in the superior colliculus and the mantle of the toral bulges. Signal was not detected in the intercollicular area.

Structure	Level	Pattern	Image	Note
TS26: inferior colliculus	Strong		10c	A series of eight dorsoventral horizontal sections show that the inferior colliculus core extends in a hook pattern, passing above the preisthmus and into the dorsomedial toral area next to the roof plate.
TS26: superior colliculus	Present	Regionally restricted	10c	Expression was detected in multiple layers in the superior colliculus.
TS26: midbrain	Strong	Regionally restricted	10c	Signal was strong in the inferior colliculus. Signal was detected in the superior colliculus and the mantle of the toral bulges. Signal was not detected in the intercollicular area.

Structure	Level	Pattern	Image	Note
TS26: inferior colliculus	Strong		10d	A series of eight dorsoventral horizontal sections show that the inferior colliculus core extends in a hook pattern, passing above the preisthmus and into the dorsomedial toral area next to the roof plate.
TS26: superior colliculus	Present	Regionally restricted	10d	Expression was detected in multiple layers in the superior colliculus.
TS26: midbrain	Strong	Regionally restricted	10d	Signal was strong in the inferior colliculus. Signal was detected in the superior colliculus and the mantle of the toral bulges. Signal was not detected in the preisthmus or the intercollicular area.

Structure	Level	Pattern	Image	Note
TS26: inferior colliculus	Strong		10e	A series of eight dorsoventral horizontal sections show that the inferior colliculus core extends in a hook pattern, passing above the preisthmus and into the dorsomedial toral area next to the roof plate.
TS26: superior colliculus	Present	Regionally restricted	10e	Expression was detected in multiple layers in the superior colliculus.
TS26: midbrain	Strong	Regionally restricted	10e	Signal was strong in the inferior colliculus, and was detected in the superior colliculus. Signal was not detected in the preisthmus or the intercollicular area.

Structure	Level	Pattern	Image	Note
TS26: inferior colliculus	Strong		10f	A series of eight dorsoventral horizontal sections show that the inferior colliculus core extends in a hook pattern, passing above the preisthmus and into the dorsomedial toral area next to the roof plate.
TS26: superior colliculus	Present	Regionally restricted	10f	Expression was detected in multiple layers in the superior colliculus.
TS26: midbrain	Strong	Regionally restricted	10f	Signal was strong in the inferior colliculus, and was detected in the superior colliculus. Signal was not detected in the intercollicular area. Some staining was observed in preisthmic patches caudal to the inferior colliculus and in the adjoining parabigeminal isthmic nucleus.

Structure	Level	Pattern	Image	Note
TS26: inferior colliculus	Strong		10g	A series of eight dorsoventral horizontal sections show that the inferior colliculus core extends in a hook pattern, passing above the preisthmus and into the dorsomedial toral area next to the roof plate.
TS26: superior colliculus	Present	Regionally restricted	10g	Expression was detected in multiple layers in the superior colliculus.
TS26: midbrain	Strong	Regionally restricted	10g	Signal was strong in the inferior colliculus, and was detected in the superior colliculus. Signal was not detected in the intercollicular area. Some staining was observed in preisthmic patches caudal to the inferior colliculus and in the adjoining parabigeminal isthmic nucleus.

Structure	Level	Pattern	Image	Note
TS26: inferior colliculus	Strong		10h	A series of eight dorsoventral horizontal sections show that the inferior colliculus core extends in a hook pattern, passing above the preisthmus and into the dorsomedial toral area next to the roof plate.
TS26: superior colliculus	Present	Regionally restricted	10h	Expression was detected in multiple layers in the superior colliculus.
TS26: midbrain	Strong	Regionally restricted	10h	Signal was strong in the inferior colliculus and brachium of the inferior colliculus nucleus, and was detected in the superior colliculus. Signal was not detected in the intercollicular area. Some staining was observed in preisthmic patches caudal to the inferior colliculus and in the adjoining parabigeminal isthmic nucleus.