Gene Expression Data
Assay Details
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Assay
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Reference:
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J:336866
Puelles L, et al., The Midbrain Preisthmus: A Poorly Known Effect of the Isthmic Organizer. Int J Mol Sci. 2023 Jun 5;24(11)
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7665230
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Gene symbol:
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Cbln2
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Gene name:
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cerebellin 2 precursor protein
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Probe:
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Cbln2_riboprobe
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Assay notes:
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The authors analyzed data from the Allen Mouse Brain and Developing Mouse Brain.
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Results
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Specimen
9g:
embryonic day 18.5
(more  )
Note: Sample was postfixed in 4% paraformaldehyde.
Specimen
9g:
(close  )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 18.5
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Sex:
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Male
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Note:
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Sample was postfixed in 4% paraformaldehyde.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: inferior colliculus |
Present |
Regionally restricted |
9g
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Expression was detected in the inferior colliculus/brachium of the inferior colliculus complex.
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| TS26: midbrain |
Present |
Regionally restricted |
9g
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The intercollicular domain appears more intensely labeled than the underlying inferior colliculus. The preisthmus was unlabeled.
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| TS26: superior colliculus |
Present |
Regionally restricted |
9g
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Several layers of the superior colliculus were labeled.
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Specimen
9h:
embryonic day 18.5
(more )
Note: Sample was postfixed in 4% paraformaldehyde.
Specimen
9h:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 18.5
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Sex:
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Male
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Note:
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Sample was postfixed in 4% paraformaldehyde.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: inferior colliculus |
Present |
Regionally restricted |
9h
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Expression was detected in the inferior colliculus/brachium of the inferior colliculus complex.
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| TS26: midbrain |
Present |
Regionally restricted |
9h
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The intercollicular domain appears more intensely labeled than the underlying inferior colliculus. The preisthmus was unlabeled.
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| TS26: superior colliculus |
Present |
Regionally restricted |
9h
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Several layers of the superior colliculus were labeled.
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| TS26: periaqueductal grey |
Strong |
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9h
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Specimen
12d:
postnatal day 56
(more )
Note: Sample was postfixed in 4% paraformaldehyde.
Specimen
12d:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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postnatal day 56
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Sex:
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Male
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Note:
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Sample was postfixed in 4% paraformaldehyde.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: inferior colliculus |
Present |
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12d
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| TS28: midbrain |
Present |
Regionally restricted |
12d
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Label is detected in parts of the inferior colliculus, but leaves the intercalated preisthmic domain wholly negative. This lack of signal can be followed from the periaqueductal gray across the intermediate
cuneiform area into the superficial derivative, the subbrachial nucleus.
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Analysis Tools