Aqp2 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:215391 Chen Y, et al., Expression of Nek1 during kidney development and cyst formation in multiple nephron segments in the Nek1-deficient kat2J mouse model of polycystic kidney disease. J Biomed Sci. 2014 Jul 17;21(1):63
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7666101
Gene symbol:	Aqp2
Gene name:	aquaporin 2

Antibody:	anti-AQP2
Detection system:	Secondary antibody coupled to horseradish peroxidase

Results

4_WT AQP2 P1 ©

Specimen 4 WT: postnatal day 1 (more

)

Structure	Level	Pattern	Image	Note
TS27: collecting duct	Present	Regionally restricted	4_WT AQP2 P1	Expressed in mature collecting ducts.

Specimen 4 -/- P1: postnatal day 1; Nek1^kat-2J/Nek1^kat-2J (more

)

Structure	Level	Pattern	Image	Note
TS27: collecting duct	Ambiguous		4_-/- AQP2 P1	No expression in mature collecting ducts. Staining for a marker of collecting tubules, Dolichos biflorus lectin, in contrast, was positive in mutant kidneys in a similar pattern when compared to wild type kidneys.

Specimen 4 -/- P7: postnatal day 7; Nek1^kat-2J/Nek1^kat-2J (more

)

Structure	Level	Pattern	Image	Note
TS28: collecting duct	Present		4_-/- AQP2 P7

Specimen 4 -/- P14: postnatal day 14; Nek1^kat-2J/Nek1^kat-2J (more

)

Structure	Level	Pattern	Image	Note
TS28: collecting duct	Present		4_-/- AQP2 P14

6Af ©

Specimen 6Af: postnatal; Nek1^kat-2J/Nek1^kat-2J (more

)

Structure	Level	Pattern	Image	Note
TS28: collecting duct	Present		6Af	Tubular cysts were seen.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support.	last database update 09/17/2024 MGI 6.24