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Gene Expression Data
Assay Details
Assay
Reference: J:201463 Watson LA, et al., Atrx deficiency induces telomere dysfunction, endocrine defects, and reduced life span. J Clin Invest. 2013 May 1;123(5):2049-63
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7705273
Gene symbol: H2ax
Gene name: H2A.X variant histone
Antibody: anti-phospho-H2A.X (Ser139)
Detection system: Secondary antibody coupled to Alexa Fluor 594
Results
Specimen 1A Ctrl: embryonic day 13.5; Foxg1tm1(cre)Skm/Foxg1+ (more )
Note: Quantification was shown in the 1A right graphs.
Structure Level Pattern Image Note
TS21: cerebral cortex Present 1A left Only a few foci (DNA damage) were seen.
Specimen 1A cKO: embryonic day 13.5; Atrxtm1Rjg/Y, Foxg1tm1(cre)Skm/Foxg1+ (more )
Note: Conditional mutant. Quantification was shown in the 1A right graphs.
Structure Level Pattern Image Note
TS21: cerebral cortex Strong 1A middle A high level of DNA damage throughout the cortex was seen.
Specimen 1A cKO;p53-/-: embryonic day 13.5; Atrxtm1Rjg/Y, Foxg1tm1(cre)Skm/Foxg1+, Trp53tm1Tyj/Trp53tm1Tyj (more )
Note: Conditional mutant. Quantification was shown in the 1A right graphs.
Structure Level Pattern Image Note
TS21: cerebral cortex Strong 1A right A high level of DNA damage throughout the cortex was seen, higher than the single cKO.
Specimen not shown Ctrl: embryonic day 13.5; Foxg1tm1(cre)Skm/Foxg1+ (more )
Note: Quantification was shown in the 1A right graphs.
Structure Level Pattern Image Note
TS21: telencephalon Present Regionally restricted Only a few foci were seen in hippocampal hem (future hippocampus) and basal ganglia.
Specimen not shown cKO: embryonic day 13.5; Atrxtm1Rjg/Y, Foxg1tm1(cre)Skm/Foxg1+ (more )
Note: Conditional mutant. Quantification was shown in the 1A right graphs.
Structure Level Pattern Image Note
TS21: telencephalon Strong Regionally restricted A high level of DNA damage throughout the hippocampal hem (future hippocampus) and basal ganglia was seen.
Specimen not shown cKO;p53-/-: embryonic day 13.5; Atrxtm1Rjg/Y, Foxg1tm1(cre)Skm/Foxg1+, Trp53tm1Tyj/Trp53tm1Tyj (more )
Note: Conditional mutant. Quantification was shown in the 1A right graphs.
Structure Level Pattern Image Note
TS21: telencephalon Strong Regionally restricted A high level of DNA damage throughout the hippocampal hem (future hippocampus) and basal ganglia was seen. Expression in the hippocampal hem was higher than the single cKO.
Specimen not shown p53-/-: embryonic day 13.5; Trp53tm1Tyj/Trp53tm1Tyj (more )
Note: Quantification was shown in the 1A right graphs.
Structure Level Pattern Image Note
TS21: cerebral cortex Present Similar as in the control.
TS21: telencephalon Present Regionally restricted Expression in the hippocampal hem (future hippocampus) and basal ganglia was similar as in the control.
Specimen 1B Ctrl: postnatal day 0.5; Foxg1tm1(cre)Skm/Foxg1+ (more )
Note: Quantification was shown in the 1B right graphs.
Structure Level Pattern Image Note
TS27: cerebral cortex Present 1B left Only a few foci were seen.
Specimen 1B cKO: postnatal day 0.5; Atrxtm1Rjg/Y, Foxg1tm1(cre)Skm/Foxg1+ (more )
Note: Conditional mutant. Quantification was shown in the 1B right graphs.
Structure Level Pattern Image Note
TS27: cerebral cortex Present 1B middle The level of DNA damage was appreciably lower in the neonatal brain. A higher level of DNA damage throughout the cortex was seen.
Specimen 1B cKO;p53-/-: postnatal day 0.5; Atrxtm1Rjg/Y, Foxg1tm1(cre)Skm/Foxg1+, Trp53tm1Tyj/Trp53tm1Tyj (more )
Note: Conditional mutant. Quantification was shown in the 1B right graphs.
Structure Level Pattern Image Note
TS27: cerebral cortex Present 1B right The level of DNA damage was appreciably lower in the neonatal brain. A higher level of DNA damage throughout the cortex was seen.
Specimen not shown Ctrl: postnatal day 0.5; Foxg1tm1(cre)Skm/Foxg1+ (more )
Note: Quantification was shown in the 1B right graphs.
Structure Level Pattern Image Note
TS27: hippocampus Present Only a few foci were seen.
Specimen not shown cKO: postnatal day 0.5; Atrxtm1Rjg/Y, Foxg1tm1(cre)Skm/Foxg1+ (more )
Note: Conditional mutant. Quantification was shown in the 1B right graphs.
Structure Level Pattern Image Note
TS27: hippocampus Present The level of DNA damage was appreciably lower in the neonatal brain. A higher level of DNA damage throughout the cortex was seen.
Specimen not shown cKO;p53-/-: postnatal day 0.5; Atrxtm1Rjg/Y, Foxg1tm1(cre)Skm/Foxg1+, Trp53tm1Tyj/Trp53tm1Tyj (more )
Note: Conditional mutant. Quantification was shown in the 1B right graphs.
Structure Level Pattern Image Note
TS27: hippocampus Present The level of DNA damage was appreciably lower in the neonatal brain. A higher level of DNA damage throughout the hippocampus was seen, higher than the single cKO.
Specimen not shown p53-/-: postnatal day 0.5; Trp53tm1Tyj/Trp53tm1Tyj (more )
Note: Quantification was shown in the 1B right graphs.
Structure Level Pattern Image Note
TS27: cerebral cortex Present Similar as in the control.
TS27: hippocampus Present Similar as in the control.
Specimen 1C Ctrl: embryonic day 13.5; Foxg1tm1(cre)Skm/Foxg1+ (more )
Note: Double labeled: red - H2ax; green - Casp3 (assay MGI:7705315). Quantification was shown in the 1C right graphs.
Structure Level Pattern Image Note
TS21: telencephalon Present 1C Ctrl right , 1C Ctrl left Only a few foci were seen, and the majority was not positive for cleaved caspase-3.
Specimen 1C cKO: embryonic day 13.5; Atrxtm1Rjg/Y, Foxg1tm1(cre)Skm/Foxg1+ (more )
Note: Conditional mutant. Double labeled: red - H2ax; green - Casp3 (assay MGI:7705315). Quantification was shown in the 1C right graphs.
Structure Level Pattern Image Note
TS21: telencephalon Present 1C cKO right , 1C cKO left The majority of cells undergoing apoptosis (i.e., cleaved caspase-3+ cells) in the cKO telencephalon also stained positive for H2ax.
Specimen not shown cKO;p53-/-: embryonic day 13.5; Atrxtm1Rjg/Y, Foxg1tm1(cre)Skm/Foxg1+, Trp53tm1Tyj/Trp53tm1Tyj (more )
Note: Conditional mutant. Double labeled: red - H2ax; green - Casp3 (assay MGI:7705315). Quantification was shown in the 1C right graphs.
Structure Level Pattern Image Note
TS21: telencephalon Trace Apoptosis (i.e., cleaved caspase-3+ cells) was rare in the cortex, hippocampal hem (future hippocampus) and basal ganglia. The majority of cells undergoing apoptosis in the cortex and basal ganglia also stained positive for H2ax.
Specimen 3C Ctrl: embryonic day 13.5; Foxg1tm1(cre)Skm/Foxg1+ (more )
Note: Quantification was shown in the 3C right graphs. Double labeled: red - H2ax; green - Pcna (assay MGI:7705325).
Structure Level Pattern Image Note
TS21: cerebral cortex Present 3C Ctrl merge , 3C Ctrl gammaH2AX Only a few foci (DNA damage) were seen.
Specimen 3C cKO: embryonic day 13.5; Atrxtm1Rjg/Y, Foxg1tm1(cre)Skm/Foxg1+ (more )
Note: Conditional mutant. Quantification was shown in the 3C right graphs. Double labeled: red - H2ax; green - Pcna (assay MGI:7705325).
Structure Level Pattern Image Note
TS21: cerebral cortex Present 3C cKO merge , 3C cKO gammaH2AX More DNA damage (H2ax+) at the late replication foci (Pcna+) compared with control cells, confirming an increase in double-stranded breaks during the replication process.
Specimen 7E Ctrl: embryonic day 13.5; Foxg1tm1(cre)Skm/Foxg1+ (more )
Structure Level Pattern Image Note
TS21: pituitary gland Present 7E Ctrl Only a few foci (DNA damage) were seen.
Specimen 7E cKO: embryonic day 13.5; Atrxtm1Rjg/Y, Foxg1tm1(cre)Skm/Foxg1+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS21: pituitary gland Present 7E cKO Increased expression compared with the control.
Specimen S1A Ctrl: postnatal day 7; Foxg1tm1(cre)Skm/Foxg1+ (more )
Structure Level Pattern Image Note
TS28: cerebral cortex Absent S1A Ctrl
Specimen S1A cKO: postnatal day 7; Atrxtm1Rjg/Y, Foxg1tm1(cre)Skm/Foxg1+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS28: cerebral cortex Absent S1A cKO
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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10/09/2024
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