Probe:	Netrin-1 probe3
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS22: thalamus mantle layer	Present	Regionally restricted	2A	Expression in differentiating mantle zone of internal capsule zone (ICZ).
TS22: thalamus ventricular layer	Present	Regionally restricted	2A	Expression in ventricular zone overlying mantle zone of ICZ.
TS22: dorsal thalamus	Present	Regionally restricted	2A	Expression in medial part of dorsal thalamus, just lateral to ventricular zone.
TS22: hippocampus	Present		2A
TS22: hypothalamus ventricular layer	Present		2A
TS22: diencephalon internal capsule	Present	Regionally restricted	2A' , 2A	Expression in cells within internal capsule zone, intermingled with fascicles of thalamocortical axons (TCAs) in internal capsule.
TS22: neocortex	Absent		2A

Structure	Level	Pattern	Image	Note
TS22: hypothalamus mantle layer	Present			At caudal levels, expression in mantle zone of ventral hypothalamus.

Structure	Level	Pattern	Image	Note
TS22: diencephalon internal capsule	Present	Regionally restricted	2B	Expression in internal capsule zone (ICZ) in cells within and surrounding internal capsule.
TS22: lateral ganglionic eminence	Present	Regionally restricted	2B	Expression in overlying ventricular zone of lateral and medial ganglionic eminences.
TS22: medial ganglionic eminence	Present	Regionally restricted	2B	Expression in overlying ventricular zone of lateral and medial ganglionic eminences.