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Gene Expression Data
Assay Details
Assay
Reference: J:176963 Sumigray KD, et al., Lis1 is essential for cortical microtubule organization and desmosome stability in the epidermis. J Cell Biol. 2011 Aug 22;194(4):631-42
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7713182
Gene symbol: Pafah1b1
Gene name: platelet-activating factor acetylhydrolase, isoform 1b, subunit 1
Antibody: anti-Lis1
Assay notes: There was another antibody from abcam mentioned in the paper. It was not specified which one was used.
Results
Specimen 2J: embryonic day 17.5 (more )
Note: Double labeled: red - Pafah1b1; green - Itgb4 (assay MGI:7713188).
Structure Cell Type Level Pattern Image Note
TS25: epidermis basal layer basal cell of epidermis Present Punctate 2J-2 , 2J Localized to a single punctum, presumably the centrosome.
TS25: dermis fibroblast of dermis Present Punctate 2J-1 , 2J Localized to a single punctum, presumably the centrosome.
TS25: epidermis suprabasal layer suprabasal keratinocyte Present 2J , 2J-3 Localized to the cell cortex, where desmosomes are abundant.
Specimen 2K: embryonic day 17.5; Dsptm1Efu/Dsptm1Efu, Tg(KRT14-cre)1Efu/0 (more )
Note: Conditional mutant. Double labeled: red - Pafah1b1; green - Itgb4 (assay MGI:7713188).
Structure Cell Type Level Pattern Image Note
TS25: epidermis suprabasal layer suprabasal keratinocyte Present Diffuse 2K-3 , 2K Localization was specifically disrupted in suprabasal cells, where it was diffuse throughout the cytoplasm.
TS25: epidermis basal layer basal cell of epidermis Present Punctate 2K-2 , 2K Remained associated with the centrosome.
TS25: dermis fibroblast of dermis Present Punctate 2K-1 , 2K Remained associated with the centrosome.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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09/17/2024
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