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Gene Expression Data
Assay Details
Assay
Reference: J:352718 Gorla M, et al., A Nedd4 E3 Ubiquitin Ligase Pathway Inhibits Robo1 Repulsion and Promotes Commissural Axon Guidance across the Midline. J Neurosci. 2022 Oct 5;42(40):7547-7561
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7716475
Gene symbol: Nedd4
Gene name: neural precursor cell expressed, developmentally down-regulated 4
Antibody: Anti-Nedd4-1 #PA5-26930
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 6D: embryonic day 11.5 (more )
Note: Double labeled: green - Nedd4; red - Robo3 (assay MGI:7716477).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord Present Regionally restricted 6D Merge , 6D Nedd4-1 Expression was detected in commissural axons. Co-localization with Robo3 indicates that expression was in both the precrossing and postcrossing portions of the commissural axons.
TS20: spinal cord spinal cord motor neuron Moderate 6D Nedd4-1 , 6D Merge
TS20: dorsal root ganglion Strong 6D Nedd4-1 , 6D Merge
Specimen 7B N4-1f/+;Wnt1Crenegative (Control): embryonic day 11.5; Nedd4tm3.1Bros/Nedd4+ (more )
Structure Level Pattern Image Note
TS20: spinal cord floor plate Strong Regionally restricted 7B left Strong expression was detected in commissural axons.
TS20: dorsal root ganglion Strong 7B left
TS20: spinal cord Present Regionally restricted 7B left Expression was detected in the dorsal spinal cord.
TS20: basal columns Moderate Regionally restricted 7B left Moderate expression was detected in the motor column.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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09/17/2024
MGI 6.24 		The Jackson Laboratory