Probe:	E13 probe
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex	Strong	Widespread	6A left	High levels of expression in dorsal pallium from ventricular to pial surface, along entire length of rostro-caudal axis.
TS20: lateral ganglionic eminence	Present	Regionally restricted	6A left	Expression in ventral pallium restricted to ventricular zone of ganglionic eminences.

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex	Strong	Widespread	6A left-middle	High levels of expression in dorsal pallium from ventricular to pial surface, along entire length of rostro-caudal axis.
TS20: lateral ganglionic eminence	Present	Regionally restricted	6A left-middle	Expression in ventral pallium restricted to ventricular zone of ganglionic eminences.
TS20: medial ganglionic eminence	Present	Regionally restricted	6A left-middle	Expression in ventral pallium restricted to ventricular zone of ganglionic eminences.

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex	Strong	Widespread	6A middle	High levels of expression in dorsal pallium from ventricular to pial surface, along entire length of rostro-caudal axis.
TS20: caudal ganglionic eminence	Present	Regionally restricted	6A middle	Expression in ventral pallium restricted to ventricular zone of ganglionic eminences.
TS20: lateral ganglionic eminence	Present	Regionally restricted	6A middle	Expression in ventral pallium restricted to ventricular zone of ganglionic eminences.

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex	Strong	Widespread	6A middle-right	High levels of expression in dorsal pallium from ventricular to pial surface, along entire length of rostro-caudal axis.
TS20: caudal ganglionic eminence	Present	Regionally restricted	6A middle-right	Expression in ventral pallium restricted to ventricular zone of ganglionic eminences.

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex	Strong	Widespread	6A right	High levels of expression in dorsal pallium from ventricular to pial surface, along entire length of rostro-caudal axis.
TS20: caudal ganglionic eminence	Present	Regionally restricted	6A right	Expression in ventral pallium restricted to ventricular zone of ganglionic eminences.

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex	Present	Graded	6B E13 mRNA hi mag , 6B E13 mRNA	Graded expression clearly visible in all cortical primordium, from ventricular (low) to pial (high) surface.
TS20: lateral ganglionic eminence	Present	Regionally restricted	6B E13 mRNA , 6B E13 mRNA hi mag	In ganglionic eminence, expression only in ventricular zone.

Structure	Level	Pattern	Image	Note
TS20: cortical preplate	Present	Graded	6B E13/NeuN hi mag , 6B E13/NeuN inset , 6B E13/NeuN	Maximum expression in post-mitotic neurons of preplate co-expressing Rbfox3.
TS20: lateral ganglionic eminence	Present	Regionally restricted	6B E13/NeuN hi mag , 6B E13/NeuN inset , 6B E13/NeuN	In ganglionic eminence, expression only in ventricular zone.

Structure	Cell Type	Level	Pattern	Image	Note
TS20: cerebral cortex subventricular zone	neural progenitor cell	Present	Graded	6B E13/Tbr2 hi mag , 6B E13/Tbr2 inset , 6B E13/Tbr2	Low levels of expression gradually increasing and co-localizing with Eomes-expressing intermediate progenitor cells of subventricular zone.
TS20: lateral ganglionic eminence		Present	Regionally restricted	6B E13/Tbr2 hi mag , 6B E13/Tbr2 inset , 6B E13/Tbr2	In ganglionic eminence, expression only in ventricular zone.

Structure	Level	Pattern	Image	Note
TS28: amygdaloid nucleus	Strong		7A
TS28: cerebral cortex	Strong		7A
TS28: hippocampus	Strong		7A

Structure	Level	Pattern	Image	Note
TS28: cingulate cortex	Strong
TS28: piriform cortex	Strong

Structure	Level	Pattern	Image	Note
TS28: primary somatosensory cortex	Present	Widespread	7B E13 mRNA , 7B E13 mRNA hi mag	In primary somatosensory cortex, widespread expression in all cortical layers.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: primary somatosensory cortex	cerebral cortex neuron	Present	Regionally restricted	7B E13/Vglut2 inset , 7B E13/Vglut1 , 7B E13/Vglut2 hi mag	High co-localization of expression with Slc17a7 in cortical neurons.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: primary somatosensory cortex	astrocyte of the cerebral cortex	Absent		7B E13/GFAP , 7B E13/GFAP hi mag , 7B E13/GFAP inset	No co-localization with Gfap-expressing mature astrogial cells..

Structure	Cell Type	Level	Pattern	Image	Note
TS28: primary somatosensory cortex	astrocyte of the cerebral cortex	Absent		7B E13/Blbp inset , 7B E13/Blbp hi mag , 7B E13/Blbp	No co-localization with Fabp7-expressing mature astrogial cells..

Structure	Cell Type	Level	Pattern	Image	Note
TS28: dentate gyrus granule cell layer	dentate gyrus granule cell	Present	Graded	7C DG E13 mRNA	Expression in glutamatergic main neurons in dentate gyrus, higher in more mature granule cells.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: dentate gyrus granule cell layer	dentate gyrus granule cell	Present		7C DG E13/Vglut1 inset , 7C DG E13/Vglut1

Structure	Cell Type	Level	Pattern	Image	Note
TS28: dentate gyrus granule cell layer	dentate gyrus granule cell	Present		7C DG E13/GFAP inset , 7C DG E13/GFAP

Structure	Cell Type	Level	Pattern	Image	Note
TS28: dentate gyrus granule cell layer	dentate gyrus granule cell	Present		7C DG E13/Blbp inset , 7C DG E13/Blbp

Structure	Cell Type	Level	Pattern	Image	Note
TS28: CA1 pyramidal cell layer	hippocampal pyramidal neuron	Present		7C CA1 E13 mRNA

Structure	Cell Type	Level	Pattern	Image	Note
TS28: dentate gyrus	dentate gyrus granule cell	Strong	Non-Uniform	7C DG E13/CALB	High levels of expression co-localized with Calb1 expression in granule cells.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: CA1 pyramidal cell layer	hippocampal pyramidal neuron	Present	Non-Uniform	7C CA1 E13/GFAP	High levels of expression co-localized with Calb1 expression in granule cells.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: hippocampus CA1	hippocampal pyramidal neuron	Present		7C CA1 E13/Blbp

Structure	Cell Type	Level	Pattern	Image	Note
TS28: hippocampus CA1	hippocampal pyramidal neuron	Present		7D E13 mRNA
TS28: hippocampus stratum oriens	hippocampal interneuron	Present		7D E13 mRNA	Expression in GABAergic interneurons in oriens and radiatum strata of hippocampus.
TS28: hippocampus stratum radiatum	hippocampal interneuron	Present		7D E13 mRNA	Expression in GABAergic interneurons in oriens and radiatum strata of hippocampus.
TS28: dentate gyrus granule cell layer	dentate gyrus granule cell	Present		7D E13 mRNA

Structure	Level	Pattern	Image	Note
TS20: brain nucleus	Present	Regionally restricted	S11A E13/TH , S11A High Mag	No co-localization with Th expression in emerging dopaminergic populations reaching final positions in developing brain.

Structure	Level	Pattern	Image	Note
TS28: ventral tegmental area	Present	Regionally restricted	S11B HIgh Mag , S11B E13/TH	No co-localization with Th expression in ventral tegmental area.