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Gene Expression Data
Assay Details
Assay
Reference: J:171072 Carre A, et al., Hes1 is required for appropriate morphogenesis and differentiation during mouse thyroid gland development. PLoS One. 2011;6(2):e16752
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7720371
Gene symbol: Nkx2-1
Gene name: NK2 homeobox 1
Antibody: anti-Nkx2-1
Detection system: Secondary antibody coupled to Alexa Fluor 633
Results
Specimen 1B: embryonic day 9.5 (more )
Structure Level Pattern Image Note
TS15: thyroid primordium Present Regionally restricted 1B Expressed in the median thyroid anlage evaginating of the endoderm.
Specimen 1C: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: thyroid gland Present Regionally restricted 1C Expressed in the median anlage.
TS19: aortic sac Present 1C
Specimen 1D: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: thyroid gland Present Regionally restricted 1D Expressed in the symmetrical ultimobranchial bodies from the endoderm of the fourth pharyngeal pouch.
Specimen 1K: embryonic day 13.5 (more )
Structure Level Pattern Image Note
TS21: thyroid gland Present 1K
Specimen 2A E9.5 +/+: embryonic day 9.5 (more )
Structure Level Pattern Image Note
TS15: thyroid primordium Present Regionally restricted 2A-1 Expressed in the thyroid median anlage within the endoderm covering the tongue.
Specimen 2A E9.5 -/-: embryonic day 9.5; Hes1tm1Fgu/Hes1tm1Fgu (more )
Structure Level Pattern Image Note
TS15: thyroid primordium Present Regionally restricted 2A-6 Reduced expression in the thyroid median anlage (smaller size) within the endoderm covering the tongue, compared with the wild-type (see Fig 2B).
Specimen 2A E11.5 +/+: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: thyroid gland Present Regionally restricted 2A-2 Expressed in the ultimobranchial bodies.
TS19: trachea epithelium Present 2A-2
Specimen 2A E11.5 -/-: embryonic day 11.5; Hes1tm1Fgu/Hes1tm1Fgu (more )
Structure Level Pattern Image Note
TS19: thyroid gland Present Regionally restricted 2A-7 Reduced expression in the ultimobranchial bodies (smaller size), compared with the wild-type (see Fig 2B).
TS19: trachea epithelium Present 2A-7
Specimen 2A E13.5 +/+: embryonic day 13.5 (more )
Structure Level Pattern Image Note
TS21: ultimobranchial body Present 2A-3
TS21: trachea epithelium Present 2A-3
Specimen 2A E13.5 -/-: embryonic day 13.5; Hes1tm1Fgu/Hes1tm1Fgu (more )
Structure Level Pattern Image Note
TS21: ultimobranchial body Present 2A-8 Reduced expression (smaller size) compared with the wild-type (see Fig 2C).
TS21: trachea epithelium Present 2A-8
Specimen 2A E15.5 +/+: embryonic day 15.5 (more )
Structure Level Pattern Image Note
TS23: thyroid gland left lobe Present 2A-4
TS23: thyroid gland right lobe Present 2A-4
TS23: trachea epithelium Present 2A-4
Specimen 2A E15.5 -/-: embryonic day 15.5; Hes1tm1Fgu/Hes1tm1Fgu (more )
Structure Level Pattern Image Note
TS23: thyroid gland left lobe Present 2A-9 Reduced expression (smaller size) compared with the wild-type (see Fig 2C).
TS23: thyroid gland right lobe Present 2A-9 Reduced expression (smaller size) compared with the wild-type (see Fig 2C).
TS23: trachea epithelium Present 2A-9
Specimen 2A E16.5 +/+: embryonic day 16.5 (more )
Structure Level Pattern Image Note
TS24: thyroid gland left lobe Present 2A-5
TS24: thyroid gland right lobe Present 2A-5
TS24: trachea epithelium Present 2A-5
Specimen 2A E16.5 -/-: embryonic day 16.5; Hes1tm1Fgu/Hes1tm1Fgu (more )
Structure Level Pattern Image Note
TS24: thyroid gland left lobe Present 2A-10 Reduced expression (smaller size) compared with the wild-type (see Fig 2C).
TS24: thyroid gland right lobe Present 2A-10 Reduced expression (smaller size) compared with the wild-type (see Fig 2C).
TS24: trachea epithelium Present 2A-10
Specimen 3A/B: embryonic day 16.5 (more )
Structure Level Pattern Image Note
TS24: thyroid gland Present Widespread 3B , 3A Expressed in all thyroid cells.
Specimen 3F/G: embryonic day 16.5; Hes1tm1Fgu/Hes1tm1Fgu (more )
Structure Level Pattern Image Note
TS24: thyroid gland Present Scattered 3F , 3G Positive cells scattered throughout the gland.
Specimen 3K/L: embryonic day 15.5 (more )
Structure Level Pattern Image Note
TS23: thyroid gland lobe Present 3L , 3K Fusion of the ultimobranchial bodies with the median anlage was apparent.
Specimen 3P/Q: embryonic day 15.5; Hes1tm1Fgu/Hes1tm1Fgu (more )
Structure Level Pattern Image Note
TS23: thyroid gland Present Regionally restricted 3P , 3Q Only smaller size positive ultimobranchial bodies were seen in the mutant.
Specimen 5A: embryonic day 16.5 (more )
Structure Level Pattern Image Note
TS24: thyroid gland Present 5A
Specimen 5D: embryonic day 16.5; Hes1tm1Fgu/Hes1tm1Fgu (more )
Structure Level Pattern Image Note
TS24: thyroid gland Present 5D
Specimen 6A/B/D: embryonic day 9.5 (more )
Note: Double labeled: red - Nkx2-1; green - BrdU.
Structure Level Pattern Image Note
TS15: thyroid primordium Present Regionally restricted 6D , 6A , 6B Expressed in the median thyroid anlage. The proportion of Nkx2-1-positive cells labelled by BrdU was 4.6% in the wild-type (see Fig 7A).
Specimen 6F/G/I: embryonic day 9.5; Hes1tm1Fgu/Hes1tm1Fgu (more )
Note: Double labeled: red - Nkx2-1; green - BrdU.
Structure Level Pattern Image Note
TS15: thyroid primordium Present Regionally restricted 6G , 6I , 6F Expressed in the median thyroid anlage. The proportion of Nkx2-1-positive cells labelled by BrdU was 13.2% in the mutant (see Fig 7A). But the number of Nkx2-1 positive progenitor cells was significantly lower (see Fig 7B).
Specimen 6K/L/N: embryonic day 15.5 (more )
Note: Double labeled: red - Nkx2-1; green - BrdU.
Structure Level Pattern Image Note
TS23: thyroid gland Present 6L , 6K , 6N
Specimen 6P/Q/S: embryonic day 15.5; Hes1tm1Fgu/Hes1tm1Fgu (more )
Note: Double labeled: red - Nkx2-1; green - BrdU.
Structure Level Pattern Image Note
TS23: thyroid gland Present 6Q , 6P , 6S The proportion of Nkx2-1-positive cells labelled by BrdU was similar as in the wild-type (see Fig 7A).
Specimen S1A +/+: embryonic day 10.5 (more )
Note: Double labeled: red - Nkx2-1; blue - Hes1 (assay MGI:7720409).
Structure Level Pattern Image Note
TS17: thyroid primordium Present S1A-3 , S1A-4 , S1A-2
Specimen S1A -/-: embryonic day 10.5; Hes1tm1Fgu/Hes1tm1Fgu (more )
Note: Double labeled: red - Nkx2-1; blue - Hes1 (assay MGI:7720409).
Structure Level Pattern Image Note
TS17: thyroid primordium Present S1A-8 , S1A-7 , S1A-6
Specimen S1B +/+: embryonic day 10.5 (more )
Note: Double labeled: red - Nkx2-1; brown - Hes1 (assay MGI:7720372).
Structure Level Pattern Image Note
TS17: thyroid primordium Present S1B-4 , S1B-2 , S1B-3
Specimen S1B -/-: embryonic day 10.5; Hes1tm1Fgu/Hes1tm1Fgu (more )
Note: Double labeled: red - Nkx2-1; brown - Hes1 (assay MGI:7720372).
Structure Level Pattern Image Note
TS17: thyroid primordium Present S1B-8 , S1B-6 , S1B-7
Specimen not shown: embryonic day 18.5 (more )
Structure Level Pattern Image Note
TS26: thyroid gland Present
Specimen not shown: postnatal adult (more )
Structure Level Pattern Image Note
TS28: thyroid gland Present
Specimen not shown: embryonic day 9.5; Hes1tm1Fgu/Hes1+ (more )
Structure Level Pattern Image Note
TS15: thyroid primordium Present Regionally restricted Expressed in the thyroid median anlage, similar level as in the wild-type.
Specimen not shown: embryonic day 11.5; Hes1tm1Fgu/Hes1+ (more )
Structure Level Pattern Image Note
TS19: thyroid gland Present Expressed in the ultimobranchial bodies, similar level as in the wild-type.
Specimen not shown: embryonic day 13.5; Hes1tm1Fgu/Hes1+ (more )
Structure Level Pattern Image Note
TS21: ultimobranchial body Present Similar level as in the wild-type.
Specimen not shown: embryonic day 15.5; Hes1tm1Fgu/Hes1+ (more )
Structure Level Pattern Image Note
TS23: thyroid gland left lobe Present Similar level as in the wild-type.
TS23: thyroid gland right lobe Present Similar level as in the wild-type.
Specimen not shown: embryonic day 16.5; Hes1tm1Fgu/Hes1+ (more )
Structure Level Pattern Image Note
TS24: thyroid gland left lobe Present Similar level as in the wild-type.
TS24: thyroid gland right lobe Present Similar level as in the wild-type.
Specimen not shown: embryonic day 11.5; Hes1tm1Fgu/Hes1tm1Fgu (more )
Note: Double labeled: red - Nkx2-1; green - BrdU.
Structure Level Pattern Image Note
TS19: thyroid gland Present The proportion of Nkx2-1-positive cells labelled by BrdU was similar as in the wild-type (see Fig 7A). But the number of Nkx2-1 positive progenitor cells was significantly lower (see Fig 7B).
Specimen not shown: embryonic day 13.5; Hes1tm1Fgu/Hes1tm1Fgu (more )
Note: Double labeled: red - Nkx2-1; green - BrdU.
Structure Level Pattern Image Note
TS21: thyroid gland Present The proportion of Nkx2-1-positive cells labelled by BrdU was similar as in the wild-type (see Fig 7A).
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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12/17/2024
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