Gene Expression Data
Assay Details
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Assay
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Reference:
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J:171072
Carre A, et al., Hes1 is required for appropriate morphogenesis and differentiation during mouse thyroid gland development. PLoS One. 2011;6(2):e16752
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7720372
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Gene symbol:
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Hes1
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Gene name:
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hes family bHLH transcription factor 1
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Antibody:
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anti-Hes1
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
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Results
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Specimen
1E/H:
embryonic day 9.5
(more  )
Specimen
1E/H:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: thyroid primordium |
Present |
Regionally restricted |
1H
,
1E
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Only expressed in a subset of Nkx2-1-positive precursors of the median anlage.
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| TS15: foregut |
Present |
Regionally restricted |
1H
,
1E
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Expressed in the thyroid anlagen surrounding tissues.
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Specimen
1F/I:
embryonic day 11.5
(more )
Specimen
1F/I:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: thyroid gland |
Present |
Regionally restricted |
1I
,
1F
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Expressed in the median anlage lining the aortic sac.
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| TS19: foregut |
Present |
Regionally restricted |
1F
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Expressed in the thyroid anlagen surrounding tissues.
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Specimen
1G/J:
embryonic day 11.5
(more )
Specimen
1G/J:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: thyroid gland |
Present |
Regionally restricted |
1G
,
1J
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Expressed in the ultimobranchial bodies in a lateral position.
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| TS19: foregut |
Present |
Regionally restricted |
1G
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Expressed in the thyroid anlagen surrounding tissues.
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Specimen
1L:
embryonic day 13.5
(more )
Specimen
1L:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: thyroid gland |
Present |
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1L
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Expressed in Ascl1/Mash1-positive cells (compared with Fig 1P).
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Specimen
1M/Q/R:
embryonic day 17.5
(more )
Note: Double labeled: brown - Hes1; red - Tg (assay MGI:7720377).
Specimen
1M/Q/R:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: brown - Hes1; red - Tg (assay MGI:7720377).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS25: thyroid gland |
thyroid follicular cell |
Present |
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1Q
,
1R
,
1M
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Expressed in TG-producing thyrocytes within thyroid follicles.
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| TS25: thyroid gland |
parafollicular cell |
Present |
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1M
,
1R
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Expressed in calcitonin-producing C-cells (compared with Fig 1N/S).
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Specimen
1O/T:
embryonic day 13.5
(more )
Specimen
1O/T:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: thyroid gland |
Present |
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1T
,
1O
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Expressed in some Nkx2-1-positive cells (compared with Fig 1K).
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Specimen
S1B +/+:
embryonic day 10.5
(more )
Note: Double labeled: brown - Hes1; red - Nkx2-1 (assay MGI:7720371).
Specimen
S1B +/+:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * CD-1
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: brown - Hes1; red - Nkx2-1 (assay MGI:7720371).
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Specimen
S1B -/-:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * CD-1
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Mutant Allele(s):
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Hes1tm1Fgu/Hes1tm1Fgu
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: brown - Hes1; red - Nkx2-1 (assay MGI:7720371).
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Specimen
not shown:
embryonic day 15.5
(more )
Specimen
not shown:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS23: thyroid gland |
thyroid follicular cell |
Present |
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Nuclear staining in most Nkx2-1-positive cells and TG-producing thyrocytes within thyroid follicles.
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| TS23: thyroid gland |
parafollicular cell |
Present |
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Expressed in calcitonin-producing C-cells.
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Specimen
not shown:
embryonic day 18.5
(more )
Specimen
not shown:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS26: thyroid gland |
thyroid follicular cell |
Present |
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Nuclear staining in most Nkx2-1-positive cells and TG-producing thyrocytes within thyroid follicles.
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Specimen
not shown:
postnatal adult
(more )
Specimen
not shown:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: thyroid gland follicle |
thyroid follicular cell |
Present |
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Nuclear staining in most Nkx2-1-positive cells and TG-producing thyrocytes within thyroid follicles.
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Analysis Tools