Probe:	Neph1 cDNA
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS22: dorsal root ganglion	Absent		1A E14.5 Neph1-/-
TS22: spinal cord	Absent		1A E14.5 Neph1-/-

Structure	Level	Pattern	Image	Note
TS21: dorsal root ganglion	Ambiguous		1A E13.5 Neph1+/+	Expression was almost undetected at E13.5.
TS21: spinal cord	Ambiguous		1A E13.5 Neph1+/+	Expression was almost undetected at E13.5.

Structure	Level	Pattern	Image	Note
TS22: dorsal root ganglion	Present		1A E14.5 Neph1+/+
TS22: spinal cord dorsal horn	Present	Regionally restricted	1A E14.5 Neph1+/+	Expression was detected in the deep dorsal horn.
TS22: spinal cord	Present	Regionally restricted	1A E14.5 Neph1+/+	Expression was detected in the ventral spinal cord.

Structure	Level	Pattern	Image	Note
TS24: dorsal root ganglion	Present		1A E16.5 Neph1+/+
TS24: spinal cord dorsal horn	Present	Regionally restricted	1A E16.5 Neph1+/+	Expression expands to the most superficial layers of the dorsal horn from E16.5 onward.

Structure	Level	Pattern	Image	Note
TS26: spinal cord dorsal horn	Present	Regionally restricted	1A E18.5 Dorsal spinal cord	Expression expands to the most superficial layers of the dorsal horn.

Structure	Level	Pattern	Image	Note
TS28: spinal cord dorsal horn	Present	Regionally restricted	1A P6 Dorsal spinal cord	Expression expands to the most superficial layers of the dorsal horn.

Structure	Level	Pattern	Image	Note
TS26: dorsal root ganglion	Present		1A E18.5 DRG

Structure	Level	Pattern	Image	Note
TS28: dorsal root ganglion	Present		1A P6 DRG

Structure	Cell Type	Level	Pattern	Image	Note
TS22: spinal cord dorsal horn	GABAergic neuron	Present	Regionally restricted	1Ba1 , 1Ba3 , 1Ba	Colocalization (arrows) with Pax2 in GABAergic neurons was extensive. Cells that did not co-localize expression were marked with asterisks.

Structure	Cell Type	Level	Pattern	Image	Note
TS22: spinal cord dorsal horn	glutamatergic neuron	Weak	Regionally restricted	1Bb , 1Bb1 , 1Bb3	Less colocalization (arrows) with Lmx1b in glutamatergic neurons was observed. Cells that did not co-localize expression were marked with asterisks.

Structure	Cell Type	Level	Pattern	Image	Note
TS22: spinal cord dorsal horn	glutamatergic neuron	Weak	Regionally restricted	1Bc1 , 1Bc , 1Bc3	Less colocalization (arrows) with Prrxl1 in glutamatergic neurons was observed. Cells that did not co-localize expression were marked with asterisks.

Structure	Level	Pattern	Image	Note
TS22: dorsal root ganglion	Present		2Da
TS22: spinal cord dorsal horn	Present		2Da

Structure	Level	Pattern	Image	Note
TS22: spinal cord	Present	Regionally restricted	2Db	Ectopic expression was observed in the mutant E14.5 superficial dorsal horn.
TS22: dorsal root ganglion	Present		2Db	No difference was observed in the dorsal root ganglion of the Prrxl1 knockout compared with wild-type.

Structure	Level	Pattern	Image	Note
TS24: dorsal root ganglion	Present		2Dc
TS24: spinal cord dorsal horn	Present		2Dc

Structure	Level	Pattern	Image	Note
TS24: dorsal root ganglion	Present		2Dd	Expression did not change in the Prrxl1 knockout as compared with wild-type embryos at E16.5.
TS24: spinal cord dorsal horn	Present		2Dd	Expression did not change in the Prrxl1 knockout as compared with wild-type embryos at E16.5.

Structure	Cell Type	Level	Pattern	Image	Note
TS22: spinal cord dorsal horn	GABAergic neuron	Present	Regionally restricted	2Ea , 2Ea1	Extensive co-localization was observed with Pax2 in GABAergic cells.
TS22: spinal cord dorsal horn	glutamatergic neuron	Weak	Regionally restricted	2Ea , 2Ea1	Less co-localization was observed with Lmx1b in glutamatergic cells.

Structure	Cell Type	Level	Pattern	Image	Note
TS22: spinal cord dorsal horn	GABAergic neuron	Weak		2Eb1 , 2Eb	Less co-localization was observed with Pax2 in GABAergic cells in the mutant.
TS22: spinal cord dorsal horn	glutamatergic neuron	Present	Regionally restricted	2Eb1 , 2Eb	In the mutant embryos, ectopic expression in the most superficial layers was extensively present in the LMX1B-positive glutamatergic cells.

Structure	Cell Type	Level	Pattern	Image	Note
TS24: spinal cord dorsal horn	GABAergic neuron	Weak	Regionally restricted	2Ec , 2Ec1	At E16.5, less colocalized expression with Pax2 was observed in the wild type dorsal horn.
TS24: spinal cord dorsal horn	glutamatergic neuron	Present	Regionally restricted	2Ec1 , 2Ec	At E16.5, more extensive colocalized expression with Lmx1b was observed in the wild type dorsal horn.

Structure	Cell Type	Level	Pattern	Image	Note
TS24: spinal cord dorsal horn	GABAergic neuron	Weak		2Ed , 2Ed1	Expression was similar to wild type mice in the mutant dorsal horn of the spinal cord at E16.5.
TS24: spinal cord dorsal horn	glutamatergic neuron	Present		2Ed , 2Ed1	Expression was similar to wild type mice in the mutant dorsal horn of the spinal cord at E16.5.

Structure	Level	Pattern	Image	Note
TS22: spinal cord cervical region	Present	Regionally restricted	S1a	Expression was detected in the dorsal cervical spinal cord.

Structure	Level	Pattern	Image	Note
TS22: spinal cord cervical region	Present	Regionally restricted	S1b	Ectopic expression was detected in the most superficial laminae of the dorsal horn of mutant cervical spinal cord.

Structure	Level	Pattern	Image	Note
TS22: spinal cord thoracic region	Present	Regionally restricted	S1c	Expression was detected in the dorsal thoracic spinal cord.

Structure	Level	Pattern	Image	Note
TS22: spinal cord thoracic region	Present	Regionally restricted	S1d	Ectopic expression was detected in the most superficial laminae of the dorsal horn of mutant thoracic spinal cord.