Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:352627
Baltar J, et al., Neph1 is required for neurite branching and is negatively regulated by the PRRXL1 homeodomain factor in the developing spinal cord dorsal horn. Neural Dev. 2024 Jul 24;19(1):13
|
|
Assay type:
|
Immunohistochemistry
|
|
MGI Accession ID:
|
MGI:7767884
|
|
Gene symbol:
|
Lmx1b
|
|
Gene name:
|
LIM homeobox transcription factor 1 beta
|
|
Antibody:
|
Anti-Lmx1b
|
|
Detection system:
|
Secondary antibody coupled to Alexa Fluor 568
|
|
|
Results
|
Specimen
1Bb2:
embryonic day 14.5
(more  )
Note: Double labeled: green - Lmx1b; magenta - Kirrel1 (assay MGI:7767882).
Specimen
1Bb2:
(close  )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: green - Lmx1b; magenta - Kirrel1 (assay MGI:7767882).
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS22: spinal cord dorsal horn |
glutamatergic neuron |
Present |
Regionally restricted |
1Bb3
,
1Bb2
|
Lmx1b is a marker for dILB glutamatergic neuronal cell population. Some co-localization was observed with Kirrel1.
|
Specimen
2Ea1:
embryonic day 14.5
(more )
Note: Triple labeled: green - Lmx1b; blue - Kirrel1 (assay MGI:7767882); magenta - Pax2 (assay MGI:7767883).
Specimen
2Ea1:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Triple labeled: green - Lmx1b; blue - Kirrel1 (assay MGI:7767882); magenta - Pax2 (assay MGI:7767883).
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS22: spinal cord dorsal horn |
glutamatergic neuron |
Present |
Regionally restricted |
2Ea1
|
Lmx1b is a marker for dILB glutamatergic neuronal cell population. Some co-localization was observed with Kirrel1.
|
Specimen
2Eb1:
(close )
|
Genetic Background:
|
involves: 129S7/SvEvBrd
|
|
Mutant Allele(s):
|
Drgxtm1And/Drgxtm1And
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Triple labeled: green - Lmx1b; blue - Kirrel1 (assay MGI:7767882); magenta - Pax2 (assay MGI:7767883).
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS22: spinal cord dorsal horn |
glutamatergic neuron |
Present |
Regionally restricted |
2Eb1
|
Lmx1b is a marker for dILB glutamatergic neuronal cell population. In the mutant embryos, ectopic expression of Kirrel1 in most superficial layers was extensively present in the Lmx1b-positive glutamatergic cells.
|
Specimen
2Ec1:
embryonic day 16.5
(more )
Note: Triple labeled: green - Lmx1b; blue - Kirrel1 (assay MGI:7767882); magenta - Pax2 (assay MGI:7767883).
Specimen
2Ec1:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 16.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Triple labeled: green - Lmx1b; blue - Kirrel1 (assay MGI:7767882); magenta - Pax2 (assay MGI:7767883).
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS24: spinal cord dorsal horn |
glutamatergic neuron |
Present |
Regionally restricted |
2Ec1
|
Lmx1b is a marker for dILB glutamatergic neuronal cell population. At E16.5, Kirrel1 co-localized more extensively with Lmx1b in the wild type dorsal horn.
|
Specimen
2Ed1:
(close )
|
Genetic Background:
|
involves: 129S7/SvEvBrd
|
|
Mutant Allele(s):
|
Drgxtm1And/Drgxtm1And
|
|
Age:
|
embryonic day 16.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Triple labeled: green - Lmx1b; blue - Kirrel1 (assay MGI:7767882); magenta - Pax2 (assay MGI:7767883).
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS24: spinal cord dorsal horn |
glutamatergic neuron |
Present |
Regionally restricted |
2Ed1
|
Lmx1b is a marker for dILB glutamatergic neuronal cell population. In the mutant at E16.5, co-localized staining with Kirrel1 was similar to wild type.
|
|
Analysis Tools