Gene Expression Data
Assay Details
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Assay
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Reference:
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J:177914
Gelman D, et al., A wide diversity of cortical GABAergic interneurons derives from the embryonic preoptic area. J Neurosci. 2011 Nov 16;31(46):16570-80
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:7865501
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Gene symbol:
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Dbx1
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Gene name:
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developing brain homeobox 1
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Reporter:
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Beta-geo
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Antibody:
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anti-betaGal
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Detection system:
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Secondary antibody coupled to Alexa Fluor 555
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Results
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Specimen
3B:
embryonic day 11.5; Dbx1tm1Tmj/Dbx1 +
(more  )
Note: Double labeled: red - Dbx1 reporter; green - Nkx6-2 (assay MGI:7865502).
Specimen
3B:
(close  )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Dbx1tm1Tmj/Dbx1+
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Dbx1 reporter; green - Nkx6-2 (assay MGI:7865502).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: diencephalon |
Present |
Regionally restricted |
3B
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Expressed in embryonic preoptic area.
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| TS19: telencephalon |
Present |
Regionally restricted |
3B
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Migration toward the pallium of the cells derived from Dbx1-expressing progenitors was seen in the medial ganglionic eminence.
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Specimen
3D:
embryonic day 11.5; Dbx1tm1Tmj/Dbx1 +
(more )
Note: Double labeled: red - Dbx1 reporter; green - Nkx2-1 (assay MGI:7865503).
Specimen
3D:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Dbx1tm1Tmj/Dbx1+
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Dbx1 reporter; green - Nkx2-1 (assay MGI:7865503).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: diencephalon |
Present |
Regionally restricted |
3D merge
,
3D left
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Expressed in the preoptic area. There were some cells coexpressed with Nkx2-1.
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Specimen
3D':
embryonic day 11.5; Dbx1tm1Tmj/Dbx1 +
(more )
Note: Double labeled: red - Dbx1 reporter; cyan - Nkx6-2 (assay MGI:7865504).
Specimen
3D':
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Dbx1tm1Tmj/Dbx1+
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Dbx1 reporter; cyan - Nkx6-2 (assay MGI:7865504).
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Specimen
not shown:
embryonic day 11.5; Dbx1tm1Tmj/Dbx1 +
(more )
Specimen
not shown:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Dbx1tm1Tmj/Dbx1+
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: diencephalon |
Present |
Regionally restricted |
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A few positive cells coexpressed Nkx6-2 at caudal levels of the preoptic area.
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Analysis Tools