How do I find the promoter (driver) that I am looking for if it is not on the selection list?
Overview
You can use the tools from the Recombinase (cre) mini homepage to access recombinase-expressing transgenes.
Find Recombinase-expressing Alleles

On most MGI pages, you can access the Recombinase (cre) Activity page from the Search menu as shown in the image at right.

You can also access the recombinase portal by clicking the Recombinase (cre) button on MGI's home page.

For this tutorial, open the Recombinase (cre) Activity page in a new window. Scroll down this page for further instructions. The "Find Recombinase-Carrying Alleles" section on the left allows you to search for recombinase expression in a specified tissue or under the control of a given promoter.
Search Menu

Example: Finding a transgene expressing recombinase under the control of HSA.
  1. Note that HSA is not listed on the Recombinase driven by selection list. Either HSA is not an approved symbol, or there are no recombinase-expressing transgenes that use this gene as a driver.

  2. Click the link to the Phenotypes, Alleles & Disease Models Query. This allows searching of synonyms.
Recombinase driven by
  1. Enter hsa in the Gene/Marker or Allele field as shown in the image on the right.

  2. Check the box next to Recombinase in the Allele attributes section.

  3. Click Search.
Allele Query Form
  1. Note that several cre-expressing transgenes are listed that have ACTA1 as a promoter. ACTA1 is the approved symbol for the gene; HSA is a synonym.

    Recombinase nomenclature takes one of two forms:

    • For randomly inserted transgenes
      Tg(Promoter-Expressed sequence)SerialNumberLabcode where
      • Tg indicates "transgene" (insertion of foreign DNA)
      • Promoter controls expression of the following sequence and takes the syntax of the species of origin (ex. ACTA1 for human, Acta1 for mouse)
      • the expressed protein follows the dash (cre or flp for the most common recombinases in mouse)
      • serial number indicates the line this allele was derived from
      • lab code indicates the research laboratory or institutional project which generated this allele.

    • For targeted transgenes
      TargetedLocustmSerialNumber(expressed sequence)Labcode where
      • targeted locus is an endogenous mouse gene (disrupted), typically lending its promoter to the expressed sequence (cre or flp recombinase) that's been introduced
      • tm indicates "targeted mutation"
      • and expressed protein, serial number and lab code are as above.

  2. Clicking on the hyperlinked allele name in the first column will bring you to the Transgene Detail page which contains information about cre activity distribution across tissues and organ systems, models which have made use of this transgene, and transgene genetic information.
Allele Summary