| Glossary Term | Immunohistochemistry |
| Definition | A method of detecting the presence of specific proteins in cells or tissues. Fixed cells or tissue on a microscope slide, made permeable if necessary with a detergent, are reacted with a primary antibody to the specific protein to be assayed. The preparation is then treated with a secondary antibody that has been coupled to an enzyme and which is directed against the primary antibody (e.g., goat anti-rabbit antibody). The preparation is then treated with a chromogenic substrate. Microscopic examination reveals the presence of staining, and hence of the specific protein to be detected. See one of the Figures from GXD for an example. |
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 11/12/2024 MGI 6.24 |
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