Symbol Name ID

Smad5 SMAD family member 5 MGI:1328787

Reference

J:183686 Lin CY, Lin CJ, Chen CH, Chen RM, Zhou B, Chang CP, The secondary heart field is a new site of calcineurin/Nfatc1 signaling for semilunar valve development. J Mol Cell Cardiol. 2012 May;52(5):1096-102

Indicates gene expression was analyzed but not necessarily detected.

Age	E13.5
Immunohistochemistry (section)
In situ RNA (section)
Immunohistochemistry (whole mount)
In situ RNA (whole mount)
In situ reporter (knock in)
Northern blot
Western blot
RT-PCR
cDNA clones
RNase protection
Nuclease S1
Primer Extension

Comments: The specificity of the antibody used was not detailed; all family members have been annotated. The antibody used recognizes the phosphorylated form of the protein.