Symbol Name ID

Shh sonic hedgehog MGI:98297

Reference

J:69771 Lewis PM, Dunn MP, McMahon JA, Logan M, Martin JF, St-Jacques B, McMahon AP, Cholesterol modification of sonic hedgehog is required for long-range signaling activity and effective modulation of signaling by Ptc1. Cell. 2001 Jun 1;105(5):599-612

Indicates gene expression was analyzed but not necessarily detected.

Age	E9.5	E10	E10.5	E11	E11.5
Immunohistochemistry (section)
In situ RNA (section)
Immunohistochemistry (whole mount)
In situ RNA (whole mount)
In situ reporter (knock in)
Northern blot
Western blot
RT-PCR
cDNA clones
RNase protection
Nuclease S1
Primer Extension

Comments: In several figures the limb buds are reported as being E10.5-10.75, so their precise age is uncertain. Age of embryo at noon of plug day not specified in reference.