Symbol Name ID

Nkx2-2 NK2 homeobox 2 MGI:97347

Reference

J:122547 Huang X, Litingtung Y, Chiang C, Region-specific requirement for cholesterol modification of sonic hedgehog in patterning the telencephalon and spinal cord. Development. 2007 Jun;134(11):2095-105

Indicates gene expression was analyzed but not necessarily detected.

Age	E10.5
Immunohistochemistry (section)
In situ RNA (section)
Immunohistochemistry (whole mount)
In situ RNA (whole mount)
In situ reporter (knock in)
Northern blot
Western blot
RT-PCR
cDNA clones
RNase protection
Nuclease S1
Primer Extension

Comments: A discrepancy between the text and the figure made it unclear which gene was examined in Fig 6. Age of embryo at noon of plug day not specified in reference.