Phenotype Image Detail

Image

Caption

Contact radiography and histology of the tibia. (A) Contact x-ray of the tibia of a 35-day-old control mouse. (B) Contact x-ray of the tibia of a 35-day-old Vdr^tm1Mbd/Vdr^tm1Mbd littermate. (C) von Kossa stain of a nondemineralized section through the tibia of the 35-day-old control mouse. (D) von Kossa stain of a nondemineralized section through the tibia of a 35-day-old Vdr^tm1Mbd/Vdr^tm1Mbd littermate. (E) Toluidine blue section through the growth plate of hte 35-day-old control mouse. (F) Toluidine blue section through the growth plate of the 35-day-old Vdr^tm1Mbd/Vdr^tm1Mbd littermate. (G) Toluidine blue section through the growth plate of a 15-day-old control mouse. (H) Toluidine blue section through the growth plate of a 15-day-old Vdr^tm1Mbd/Vdr^tm1Mbd littermate. Consistent with rickets, the tibia in the 35-day-old Vdr^tm1Mbd/Vdr^tm1Mbd mouse shows decreased cortial width along the diaphysis (arrowhead) as well as expansion and flaring of the growth plate (arrow) (Panel B) as compared to the control littermate (Panel A). Histological analyses of the skeleton of same mutant mice shows decreased mineralization (mineral stains black) (Panel D) in comparison with the control (Panel C). Toluidine blue section through growth plate of the mutant indicates marked disorganization with and increase in vascularity (arrows), matrix (arrowhead) and zone of hypertrophic chondrocytes (Panel F), as compared to the control (Panel E). Histology of the growth plate of a 15-day-old mutant indicates a 15% increase in the number of hypertrophic chondrocytes per column (Panel H, bracketed) compared to control (Panel G, bracketed).

Copyright

This image is from Li YC, Proc Natl Acad Sci U S A 1997 Sep 2;94(18):9831-5. Copyright 1997 National Academy of Sciences, U.S.A. J:42815

Associated Alleles

Symbol	Name
Vdr^tm1Mbd	vitamin D (1,25-dihydroxyvitamin D3) receptor; targeted mutation 1, Marie B Demay

Associated Genotypes

Allelic Composition	Genetic Background
Vdr^tm1Mbd/Vdr^tm1Mbd	involves: 129S4/SvJae