Phenotype Image Detail

Image

Caption

Paraffin (5 um) sections from whole eye stained with hematoxylin and eosin. Abbreviations: retinal pigment epithelium (RPE); outer segments (OS); inner segments (IS); outer nuclear layer (ONL); inner nuclear layer (INL); ganglion cell layer (GCL). A: At P8, the thickness of the outer nuclear layer was comparable between the Aipl1^tm1Visu/Aipl1^tm1Visu and wild-type littermates. B: By P18, a single row of nuclei in the ONL are present in the homozygous mutants compared to 10-12 rows of nuclei in the heterozygous and wild-type littermates. C: At P30, the degeneration of photoreceptors is complete with no ONL. The INL and GCL appear to be unaffected. For clarity, mutant is shown at a x100 magnification compared to wild-type littermate, which is shown at a x20 magnification. D: Immunostaining of P10 retinal sections with antibody against rod opsin (red) shows the normal formation of rods in homozygotes. The distribution of rhodopsin appears to be normal in homozygotes. E: Staining of retinal sections from P10 with peanut agglutinin (PNA) conjugated to FITC (green) show the formation of cones in homozygous mice. F: Immunostaining of P18 retinal sections with antibody against rod opsin (red) shows the rapid rod cell degeneration in homozygous mice. Only a single layer of opsin positive layer (red) is found in the retinal section from the homozygous mutant mouse. G: Staining of retinal sections from P18 with peanut agglutinin (PNA) conjugated to FITC (green) shows the rapid degeneration of cones in the Aipl1^tm1Visu/Aipl1^tm1Visu mice. Few cone cells (green) are found in the retinal section from the homozygous mutant mice. Increased PNA staining in cone pedicles of wild-type retinal section was seen in P18 in comparison to wild-type retinal section from P10 (g and e). In d-g, cell nuclei were stained with 4',6'-diamidino-2-phenylindole (blue).

Copyright

This image is from Ramamurthy V, Proc Natl Acad Sci U S A 2004 Sep 21;101(38):13897-902. Copyright 2004 National Academy of Sciences, U.S.A. J:92617

Associated Alleles

Symbol	Name
Aipl1^tm1Visu	aryl hydrocarbon receptor-interacting protein-like 1; targeted mutation 1, Visvanathan Ramamurthy

Associated Genotypes

Allelic Composition	Genetic Background
Aipl1^tm1Visu/Aipl1^tm1Visu	involves: 129S1/Sv * 129X1/SvJ * C57BL/6J