Phenotype Image Detail

Image

Caption

Histological analysis of long bones of Tg(Col2a1-PTHLH)1Ecw/0 (B-F, H & I) and normal mice (A & G). All sections were nondecalcified and stained with toluidine blue. A-C: Photomicrographs of the femur of normal (A) and transgenic (B & C) neonatal mice at low (A & B) and high magnification (C). D: Tibia of transgenic mouse at 1 week of age. Contrary to the normal pattern of endochondral ossification, chondrocyte differentiation is beginning around the periphery of the long bone, as indicated by the presence of prehypertrophic and hypertrophic chondrocytes. E: Section of femur at 2 weeks of age. Primary spongiosa at the periphery and persistent cartilaginous core are evident, further indicating a reversal in the normal program of ossification. F: Femur of transgenic mice at 3 weeks. Skeletal tissue has largely been replaced by woven bone. Irregular areas of cartilage, persist, forming a highly irregular pseudogrowth plate. Cortex and marrow cavity are absent. G: Femur of a nontransgenic mouse at 3 weeks of age. Note the cortex, marrow cavity, and growth plate. H: High-power photomicrograph of primary spongiosa in the femur of a 3 week old transgenic mouse. Osteblasts (red arrows), osteoclasts (yellow arrows) and osteoid are abundant, as are fibroblasts and fibrous tissue (green arrows). I: Femur of a transgenic mouse at 7 weeks of age. The long bone has essentially normalized, except for some residual irregularity and thickening in the growth plate. Magnification: A,B,D-G & I: x14; C: x45; H x224

Copyright

This image is from Weir EC, Proc Natl Acad Sci U S A 1996 Sep 17;93(19):10240-5. Copyright 1996 National Academy of Sciences, U.S.A. J:76983

Associated Alleles

Symbol	Name
Tg(Col2a1-PTHLH)1Ecw	transgene insertion 1, Eleanor C Weir

Associated Genotypes

Allelic Composition	Genetic Background
Tg(Col2a1-PTHLH)1Ecw/0	involves: C57BL/6 * SJL