Phenotype Image Detail

Image

Caption

A: Pancreatic sections from wild-type and Cyb5r4^tm1Hfb/Cyb5r4^tm1Hfb male mice at 4, 7 and 21 weeks of age that are stained in situ using anti-insulin antibody for beta cells and anti-glucagon antibody for alpha cells. The immunostains are shown in brown color. B: Electron micrographs from 7-week-old male wild-type and homozygous mutant mice. (a) In wild type islets, well granulated beta cells are the predominant islet cell type. Alpha cells are less numerous. (b) Dearth of beta cells in a homozygous mutant islet. The beta cell in the field is heavily degranulated. Mitochondria are increased in number and size. Golgi stacks are prominent, but beta granules are reduced in number primarily associated with the plasma membrane (emiocytosis). (c) Electron-dense inclusions (arrows) are present within some mitochondria in an islet from the same homozygous mutant mouse shown in b. Abbreviations: Alpha: (alpha cells); Beta: (beta cells); Mitochondria: (M); Golgi stacks: (G); beta granules: (GR)

Copyright

This image is from Xie J, Proc Natl Acad Sci U S A 2004 Jul 20;101(29):10750-5. Copyright 2004 National Academy of Sciences, U.S.A. J:91486

Associated Alleles

Symbol	Name
Cyb5r4^tm1Hfb	cytochrome b5 reductase 4; targeted mutation 1, H Franklin Bunn

Associated Genotypes

Allelic Composition	Genetic Background
Cyb5r4^tm1Hfb/Cyb5r4^tm1Hfb	involves: 129S4/SvJae * BALB/cAnN