Phenotype Image Detail

Image

Caption

A-E: Toluidine blue-stained sections of the gracile fasciculus (posterior column for hind limb) at the fifth cervical segment of the spinal cord of a wild-type mouse on a normal diet (A), an Ttpa^tm1Hsz/Ttpa^tm1Hsz mouse on an alphha-tocopherol-deficient diet (B) a Ttpa^tm1Hsz/Ttpa^tm1Hsz mouse on a normal diet (C), a Ttpa^tm1Hsz/Ttpa^tm1Hsz|0) mouse with alpha-tocopherol supplementation (D), and a wild-type mouse on an alpha-tocopherol-deficient diet (E). Marked reduction of myelinated fibers is seen in the gracile fasciculus of the homozygous mutant mice. The change was more prominent for those on a deficient diet (B), improved dramatically by supplementation of alpha-tocopherol (D), and mild in wild-type mice on an alpha-tocopherol deficient diet (E). F-H: Gliosis in the gracile fasciculus and nucleus at the medulla. I-K: Gliosis in the anterior horn of the fifth cervical segment of the spinal cord. L-N: Small angulated atrophic fibers (arrows) in the quadriceps femoris muscle. Wild-type mouse on a normal diet(F,I & L), homozygous mutant mouse on a normal diet (G,J & M), and homozygous mutant mouse with alpha-tocopherol supplementation (H,K & N). Immunohistochemistry with anti-antiglial fibrillary acidic protein antibody (F-K) and stain with hematoxylin/eosin (L-N, O, and P). Shown also are retinal sections from wild-type mouse (O) and homozygous mouse (P) on a normal diet stained with hematoxylin/eosin. The thicknesses of the outer nuclear layer (ONL) and the inner (IS) and outer (OS) segments of photoreceptor cells were reduced. GCL, ganglion cell layer; IPL, inner plexiform layer; OPL, outer plexiform layer. Scalebars: A-E, 25um; I-K, 100um; L-N, 50um

Copyright

This image is from Yokota T, Proc Natl Acad Sci U S A 2001 Dec 18;98(26):15185-90. Copyright 2001 National Academy of Sciences, U.S.A. J:90006

Associated Alleles

Symbol	Name
Ttpa^tm1Hsz	tocopherol (alpha) transfer protein; targeted mutation 1, Hiroshi Suzuki

Associated Genotypes

Allelic Composition	Genetic Background
Ttpa^tm1Hsz/Ttpa^tm1Hsz	involves: 129S7/SvEvBrd * C57BL/6J